L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages.

L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages. Research Abstract Details 

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L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages. Abstract Text:

Nanchaya Wanasen,Carol L MacLeod,Lesley G Ellies,Lynn Soong,

Leishmania spp. are obligate intracellular parasites, requiring a suitable microenvironment for their growth within host cells. We previously reported that the growth of Leishmania amazonensis amastigotes in murine macrophages (Mphis) was enhanced in the presence of gamma interferon (IFN-gamma), a Th1 cytokine normally associated with classical Mphi activation and killing of intracellular pathogens. In this study, we provided several lines of evidence suggesting that IFN-gamma-mediated parasite growth enhancement was associated with L-arginine transport via mouse cationic amino acid transporter 2B (mCAT-2B). (i) mRNA expression of Slc7A2, the gene encoding for mCAT-2B, as well as L-arginine transport was increased in IFN-gamma-treated Mphis. (ii) Supplementation of L-arginine in Mphi cultures increased parasite growth. (iii) Parasite growth enhancement in wild-type Mphis was inhibited in the presence of nonmetabolized L-arginine analogues. (iv) IFN-gamma-mediated parasite growth was absent in Mphis derived from mCAT-2B-deficient mice. Although we detected a clear upregulation of mCAT-2B and L-arginine transport, no measurable iNOS or arginase activities were observed in IFN-gamma-treated, infected Mphis. Together, these data suggest an involvement of a novel L-arginine usage independent of iNOS and arginase activities during IFN-gamma-mediated parasite growth enhancement. A possible role of mCAT-2B in supplying L-arginine directly to the parasites for their proliferation is discussed.

L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages. Publishing Authors By Initials

N Wanasen,CL MacLeod,LG Ellies,L Soong,

For similar animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred balb c research abstracts see: animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred balb c research

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L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Infection and immunity

VOLUME: 75

Page Numbers: 2802-10

Journal Abbreviation: Infect. Immun.

ISSN: 0019-9567

DAY: 26

MONTH: 03

YEAR: 2007

L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages. Keywords Mesh Terms:

KEYWORDS: Mice, Inbred BALB C

MESH TERMS: parasitology

Chemical & Substance for Abstract: L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages. Information

Substance Name: Arginine

Registry Number: 74-79-3

Grant and Affiliation Information for L-arginine and cationic amino acid transporter 2B regulate growth and survival of Leishmania amazonensis amastigotes in macrophages.

AFFILIATION: Department of Microbiology and Immunology, University of Texas Medical Branch, Medical Research Building, Rm. 3.132, 301 University Boulevard, Galveston, TX 77555-1070, USA.

Country: United States

AGENCY: United States NCI

GRANT: K08 CA 88035

ACRONYM: CA

MEDLINETA: Infect Immun

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