Kruppel-like Factor 4 Is Acetylated by p300 and Regulates Gene Transcription via Modulation of Histone Acetylation.

Kruppel-like Factor 4 Is Acetylated by p300 and Regulates Gene Transcription via Modulation of Histone Acetylation. Research Abstract Details 

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Kruppel-like Factor 4 Is Acetylated by p300 and Regulates Gene Transcription via Modulation of Histone Acetylation. Abstract Text:

Paul M Evans,Wen Zhang,Xi Chen,Jun Yang,Kishor K Bhakat,Chunming Liu,Paul M Evans,Wen Zhang,Xi Chen,Jun Yang,Kishor K Bhakat,Chunming Liu,Paul M Evans,Wen Zhang,Xi Chen,Jun Yang,Kishor K Bhakat,Chunming Liu,

Colon cancer is the second leading cause of cancer death in the United States. Krüppel-like factor 4 (KLF4) is a transcription factor involved in both proliferation and differentiation in the colon. It is down-regulated in both mouse and human colonic adenomas and has been implicated as a tumor suppressor in the gut, whereas in breast cancer, KLF4 is an oncogene. KLF4 is also involved in reprogramming differentiated cells into pluripotent stem cells. KLF4 can act as a transcriptional activator or repressor, but the underlying mechanisms are poorly understood. We found that p300, a CREB-binding protein-related protein, interacts with KLF4 both in vitro and in vivo and activates transcription. We further made the novel observation that endogenous KLF4 is acetylated by p300/CBP in vivo and that mutations of the acetylated lysines resulted in a decreased ability of KLF4 to activate target genes, suggesting that acetylation is important for KLF4-mediated transactivation. Furthermore, we found that KLF4 differentially modulates histone H4 acetylation at the promoters of target genes. Co-transfection of KLF4 and HDAC3 resulted in a synergistic repression of a cyclin B(1) reporter construct. Our results suggest that KLF4 might function as an activator or repressor of transcription depending on whether it interacts with co-activators such as p300 and CREB-binding protein or co-repressors such as HDAC3.

Kruppel-like Factor 4 Is Acetylated by p300 and Regulates Gene Transcription via Modulation of Histone Acetylation. Publishing Authors By Initials

PM Evans,W Zhang,X Chen,J Yang,KK Bhakat,C Liu,PM Evans,W Zhang,X Chen,J Yang,KK Bhakat,C Liu,PM Evans,W Zhang,X Chen,J Yang,KK Bhakat,C Liu,

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Kruppel-like Factor 4 Is Acetylated by p300 and Regulates Gene Transcription via Modulation of Histone Acetylation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 33994-4002

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 1

MONTH: 10

YEAR: 2007

Kruppel-like Factor 4 Is Acetylated by p300 and Regulates Gene Transcription via Modulation of Histone Acetylation. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

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Grant and Affiliation Information for Kruppel-like Factor 4 Is Acetylated by p300 and Regulates Gene Transcription via Modulation of Histone Acetylation.

AFFILIATION: Sealy Center for Cancer Cell Biology, the Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555.

Country: United States

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MEDLINETA: J Biol Chem

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