Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells.

Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. Research Abstract Details 

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Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. Abstract Text:

Tae Bong Kang,Yung Choon Yoo,Kwan Hee Lee,Ho Sup Yoon,Erk Her,Jong Bae Kim,Seong Kyu Song,

Synthesis of nitric oxide (NO) is one of the important effector functions of innate immune cells. Although several reports have indicated mistletoe lectins induce immune cells to produce cytokines, studies regarding the activities of the lectins in the production of NO have been very limited. Here, we report on the induction of NO synthesis in a murine macrophage cell line, RAW264.7, by Korean mistletoe lectin (KML-IIU). When the macrophage cells were treated with KML-IIU in the presence of a suboptimal concentration of IFN-gamma, NO production was induced in a concentration-dependent manner. Significantly higher levels of NO were induced by subchains of the KML-IIU (A and B), which have lower toxicities, as compared to the hololectin. Furthermore, expression of the inducible nitric oxide synthase (iNOS) gene was elevated in accordance with the level of NO production. When the synthase was inhibited by iNOS inhibitors (L-NIL and L-NAME), NO production was specifically reduced in a concentration-dependent manner. Our studies demonstrate that the KML-IIU and its subchains induce NO production in murine macrophage cells via activation of the iNOS gene expression, suggesting that the KML-IIU subchains may be used as an immunomodulator to enhance the effector functions of innate immune cells.

Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. Publishing Authors By Initials

TB Kang,YC Yoo,KH Lee,HS Yoon,E Her,JB Kim,SK Song,

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Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical science

VOLUME: 15

Page Numbers: 197-204

Journal Abbreviation: J. Biomed. Sci.

ISSN: 1423-0127

DAY: 18

MONTH: 10

YEAR: 2007

Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. Information

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LANGUAGE: eng

NlmUniqueID: 9421567

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Grant and Affiliation Information for Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells.

AFFILIATION: School of Food and Biosciences, Handong Global University, Pohang, 791-708, Korea.

Country: Netherlands

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MEDLINETA: J Biomed Sci

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