Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro.

Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Research Abstract Details 

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Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Abstract Text:

X He,M Pool,K M Darcy,S B Lim,N Auersperg,J S Coon,W T Beck,

Polypyrimidine tract-binding protein (PTB) is an RNA-binding protein with multiple functions in the regulation of RNA processing and IRES-mediated translation. We report here overexpression of PTB in a majority of epithelial ovarian tumors revealed by immunoblotting and tissue microarray (TMA) staining. By western blotting, we found that PTB was overexpressed in 17 out of 19 ovarian tumor specimens compared to their matched-normal tissues. By TMA staining, we found PTB expression in 38 out of 44 ovarian cancer cases but only in two out of nine normal adjacent tissues. PTB is also overexpressed in SV40 large T-antigen immortalized ovarian epithelial cells compared to normal human ovarian epithelial cells. Using doxycycline-inducible small interfering RNA technology, we found that knockdown of PTB expression in the ovarian tumor cell line A2780 substantially impaired tumor cell proliferation, anchorage-independent growth and in vitro invasiveness. These results suggest that overexpression of PTB is an important component of the multistep process of tumorigenesis, and might be required for the development and maintenance of epithelial ovarian tumors. Moreover, because of its novel role in tumor cell growth and invasiveness, shown here for the first time, PTB may be a novel therapeutic target in the treatment of ovarian cancer.

Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Publishing Authors By Initials

X He,M Pool,KM Darcy,SB Lim,N Auersperg,JS Coon,WT Beck,

For similar investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: tissue array analysis research abstracts see: investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: tissue array analysis research

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Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Oncogene

VOLUME: 26

Page Numbers: 4961-8

Journal Abbreviation: Oncogene

ISSN: 0950-9232

DAY: 19

MONTH: 02

YEAR: 2007

Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 8711562

Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Keywords Mesh Terms:

KEYWORDS: Tissue Array Analysis

MESH TERMS: metabolism

Chemical & Substance for Abstract: Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Information

Substance Name: Polypyrimidine Tract-Binding Protein

Registry Number: 139076-35-0

Grant and Affiliation Information for Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro.

AFFILIATION: Gynecologic Oncology Group, Core Laboratory in Molecular Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA.

Country: England

AGENCY: United States NCRR

GRANT: C06RR15482

ACRONYM: RR

MEDLINETA: Oncogene

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