Kinetic analysis of the effects of translation enhancers in translation initiation.

Kinetic analysis of the effects of translation enhancers in translation initiation. Research Abstract Details 

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Kinetic analysis of the effects of translation enhancers in translation initiation. Abstract Text:

Shuntaro Takahashi,Hiroyuki Furusawa,Yoshihiro Shimizu,Takuya Ueda,Yoshio Okahata,

Translation initiation is the most important step within a series of protein biosynthesis processes because the incorporation of ribosomes to a mRNA mainly determines efficiencies of translation. In bacteria, translation enhancers located on the 5' upstream of the Shine-Dargalno (SD) sequence on mRNAs are known to accelerate the efficiency of protein biosynthesis. To investigate the role of translation enhancers in translation initiation, we analyzed binding kinetics of a 30S ribosomal subunit to a mRNA immobilized on a 27 MHz quartz-crystal microbalance (QCM). The association constant (Ka) was rather low for the mRNA including a translation enhancer sequence compared with that for the mRNA without translation enhancers. These kinetic parameters suggest that translation enhancers destabilize the ribosome-mRNA complex on an SD sequence to move on the next step of decoding its mRNA.

Kinetic analysis of the effects of translation enhancers in translation initiation. Publishing Authors By Initials

S Takahashi,H Furusawa,Y Shimizu,T Ueda,Y Okahata,

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Kinetic analysis of the effects of translation enhancers in translation initiation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nucleic acids symposium series (2004)

VOLUME:

Page Numbers: 45-6

Journal Abbreviation: Nucleic Acids Symp Ser (Oxf)

ISSN: 1746-8272

DAY: 21

MONTH: 11

YEAR: 2007

Kinetic analysis of the effects of translation enhancers in translation initiation. Information

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LANGUAGE: eng

NlmUniqueID: 101259965

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Grant and Affiliation Information for Kinetic analysis of the effects of translation enhancers in translation initiation.

AFFILIATION: Department of Biomolecular Engineering, Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.

Country: England

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MEDLINETA: Nucleic Acids Symp Ser (Oxf)

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