Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity.

Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity. Research Abstract Details 

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Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity. Abstract Text:

Yasuhiko Hashida,Kuniyo Inouye,Yasuhiko Hashida,Kuniyo Inouye,

Thermolysin activity as well as its stability is remarkably enhanced by high concentration of neutral salts consisting of Na(+), K(+), Cl(-) and Br(-) in the synthesis and hydrolysis of N-carbobenzoxy-L-aspertyl-L-phenylalanine methyl ester and hydrolysis of N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide (FAGLA) [Inouye, K. (1992) J. Biochem. 112, 335-340]. However, effect of divalent salts on thermolysin activity has not been investigated systematically. In this study, effect of Co(2+) ion on thermolysin activity in the hydrolysis of FAGLA was examined. Thermolysin activity increased 3-4 times with increasing the Co(2+) concentration to 2 mM, but the enhanced activity was considerably reduced with higher Co(2+) concentration (2-18 mM). The activation-and-inhibition dual effects of Co(2+) ion were analysed kinetically. Release of the catalytic Zn(2+) ion from thermolysin, concomitantly occurred with the Co(2+)-dependent activation, was measured with a Zn(2+)-specific fluorescent probe. This indicates that the activation is caused by substituting Co(2+) ion for the catalytic Zn(2+) ion. Meanwhile, the Co(2+)-dependent activation was inhibited competitively by Zn(2+) ion (0.1-1.0 muM) added, similarly to that it is inhibited by higher concentration of Co(2+) ion. These lines of evidence provide a strategy for regulating thermolysin activity with Co(2+) and Zn(2+) ions.

Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity. Publishing Authors By Initials

Y Hashida,K Inouye,Y Hashida,K Inouye,

For similar inorganic chemicals: zinc compounds research abstracts see: inorganic chemicals: zinc compounds research

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Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 141

Page Numbers: 843-53

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 3

MONTH: 04

YEAR: 2007

Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity. Keywords Mesh Terms:

KEYWORDS: Zinc Compounds

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity. Information

Substance Name: Thermolysin

Registry Number: EC 3.4.24.27

Grant and Affiliation Information for Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity.

AFFILIATION: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

Country: Japan

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MEDLINETA: J Biochem (Tokyo)

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