Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export.

Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. Research Abstract Details 

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Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. Abstract Text:

J Stader,S A Benson,T J Silhavy,

We have developed a quantitative assay to measure the rate of processing of precursor LamB into mature protein and have used this assay to characterize 10 previously isolated and 3 new lamB signal sequence mutants. The data suggest that the LamB signal sequence serves a complex function. Our assay has revealed five types of signal sequence defect: 1) a strong kinetic defect resulting from alteration of the secondary structure in the putative alpha-helical region in the hydrophobic core, 2) a strong, or 3) a weak kinetic defect due to placement of a charged residue in the hydrophobic core, 4) decreased synthesis of LamB, and 5) both a decrease in synthesis and a strong kinetic defect. The effect of an extragenic suppressor, prlA4 on the rate of processing pLamB containing signal sequence mutations was also examined and compared to the rates in wild-type strains. It was found that prlA4 increases the rate of processing in some, but not all, mutants having a kinetic defect while having no effect on the decreased synthesis seen in mutants of types 4 and 5.

Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. Publishing Authors By Initials

J Stader,SA Benson,TJ Silhavy,

For similar proteins: membrane proteins: receptors, cell surface: receptors, virus research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, virus research

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Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 261

Page Numbers: 15075-80

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 15

MONTH: Nov

YEAR: 1986

Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. Keywords Mesh Terms:

KEYWORDS: Receptors, Virus

MESH TERMS: genetics

Chemical & Substance for Abstract: Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. Information

Substance Name: maltoporins

Registry Number: 0

Grant and Affiliation Information for Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export.

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Country: UNITED STATES

AGENCY: United States NIGMS

GRANT: GM34821

ACRONYM: GM

MEDLINETA: J Biol Chem

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Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export Related Publications

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• Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export.
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