Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture.

Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture. Abstract Text:

Amy S Colwell,Rui Yun,Thomas M Krummel,Michael T Longaker,H Peter Lorenz,

BACKGROUND: The mechanism of fetal scarless wound repair is poorly understood but is thought to involve unique characteristics and behavior patterns of the fetal dermal fibroblast. The authors hypothesized that keratinocytes may differentially modulate expression of key growth factors expressed during wound healing in fetal and postnatal fibroblasts. METHODS: Murine E17 fetal (n = 12 animals) and newborn (n = 8 animals) fibroblasts were grown in isolation and co-culture with newborn keratinocytes (n = 12 animals). Quantitative real-time polymerase chain reaction was performed for transforming growth factor (TGF)-beta isoform, receptor, and signaling molecule (Smad) gene expression in each group under both conditions. RESULTS: At baseline, fetal fibroblasts have 1.8-fold greater TGF-beta3 expression than postnatal fibroblasts (p < 0.01). Keratinocytes induce a further increase of TGF-beta3 expression (p < 0.01) but decreased TGF-beta1, TGF-beta2, TGF-beta receptor (R)-I, and TGF-betaR-II expression in fetal fibroblasts. Keratinocytes also induce an increase in TGF-beta3 (p < 0.01) and a decrease TGF-beta2, TGF-betaR-I, and TGF-betaR-II expression in postnatal fibroblasts; however, TGF-beta1 expression is unchanged. Fetal fibroblasts have lower baseline expression of Smad3 and Smad4 than postnatal fibroblasts (p < 0.05). Keratinocytes decrease Smad3 and increase Smad7 expression in both fetal and postnatal fibroblasts (p < 0.01). In contrast, keratinocytes decrease Smad2 only in fetal fibroblasts (p < 0.05). CONCLUSIONS: Keratinocytes have an overall antifibrotic influence on both fetal and postnatal fibroblasts in co-culture conditions. These data further characterize intrinsic differences between fetal and postnatal fibroblasts.

Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture. Publishing Authors By Initials

AS Colwell,R Yun,TM Krummel,MT Longaker,HP Lorenz,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Plastic and reconstructive surgery

VOLUME: 119

Page Numbers: 1440-5

Journal Abbreviation:

ISSN: 1529-4242

DAY: 15

MONTH: Apr

YEAR: 2007

Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1306050

Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture. Information

Substance Name: Transforming Growth Factor beta

Registry Number: 0

Grant and Affiliation Information for Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture.

AFFILIATION: Department of Surgery, Division of Plastic Surgery, Children's Surgical Research Program, Tissue Regeneration Laboratory, Stanford, CA 94305-5148, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01GM041343

ACRONYM: GM

MEDLINETA: Plast Reconstr Surg

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture Related Publications

• A systematic review of measures of end-of-life care and its outcomes.
• Adult and fetal wound healing.
• Airway management in a 980-lb patient: use of the Aintree intubation catheter.
• Autoantigens are translocated into small apoptotic bodies during early stages of apoptosis.
• Cyclophilin C-associated protein is up-regulated during wound healing.
• Electron microscopic findings in levator muscle biopsies of patients with isolated congenital or acquired ptosis.
• Engulfment of apoptotic cells: signals for a good meal.
• Epithelial-mesenchymal transition occurs after epidermal development in mouse skin.
• Familial early onset sarcoidosis with bone cysts and erosions.
• Feeding, nutrient flow, and functional gut morphology in the cricket Gryllus bimaculatus.
• Juvenile hormonal activity of mono- and polyenehomobenzenes in Drosophila virilis, Spodoptera frugiperda and Araschnia levana.
• Keratinocytes modulate fetal and postnatal fibroblast transforming growth factor-beta and Smad expression in co-culture.
• Morbid anatomy, histopathology, and histopathogenesis of fore-stomach carcinoma in mice fed carcinogenic hydrocarbons in oil emulsions.
• Novel targets for antibiotics in Staphylococcus aureus.
• Oogenesis-flight syndrome in crickets: age-dependent egg production, flight performance, and biochemical composition of the flight muscles in adult female Gryllus bimaculatus.
• Protease inhibitors bacterial proteases and their inhibitors.
• Protease inhibitors, assay and nature of serum antiprotease.
• Quality of life diagnosis and therapy as complex intervention for improvement of health in breast cancer patients: delineating the conceptual, methodological, and logistic requirements (modeling).
• Quantitative histologic analysis of the effect of chronic whole-body irradiation with gamma rays on the spermatogenic elements and the interstitial tissue of the testes of mice.
• Retention of radon by the mouse; experimental determination of biodecay and energy absorbed.
• The hematological effects of ionizing radiations.
• The potential of the patient room to promote healing and well-being in patients and nurses: an integrative review of the research.
• [Epidemiology, diagnosis, and treatment of giant pleomorphic adenomas of the parotid gland]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [VITAMIN A ESTER, FREE VITAMIN A AND CAROTENOIDS IN THE SERUM OF NORMAL ADULTS. CONTRIBUTION TO MICRO-DETERMINATION BY COLUMN CHROMATOGRAPHY.]