Keratin mutation primes mouse liver to oxidative injury.

Keratin mutation primes mouse liver to oxidative injury. Research Abstract Details 

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Keratin mutation primes mouse liver to oxidative injury. Abstract Text:

Mutation of the cytoskeletal intermediate filament proteins keratin 8 and keratin 18 (K8/K18) is associated with cirrhosis in humans, whereas transgenic mice that overexpress K18 Arg89-->Cys (R89C) have significant predisposition to liver injury. To study the mechanism of keratin-associated predisposition to liver injury, we used mouse microarrays to examine genetic changes associated with hepatocyte keratin mutation and assessed the consequences of such changes. Liver gene expression was compared in R89C versus nontransgenic or wild-type K18-overexpressing mice. Microarray-defined genetic changes were confirmed by quantitative polymerase chain reaction. Nineteen genes had a more than two-fold altered expression (nine downregulated, 10 upregulated). Upregulated genes in keratin-mutant hepatocytes included the oxidative metabolism genes cytochrome P450, S-adenosylhomocysteine (SAH) hydrolase, cysteine sulfinic acid decarboxylase, and oxidation-reduction pathway genes. Downregulated genes included fatty acid binding protein 5, cyclin D1, and some signaling molecules. Several methionine metabolism-related and glutathione synthetic pathway intermediates, including S-adenosylmethionine (SAMe) and SAH, were modulated in R89C versus control mice. R89C livers had higher lipid and protein oxidation by-products as reflected by increased malondialdehyde and oxidized albumin. In conclusion, K18 point mutation in transgenic mice modulates several hepatocyte oxidative stress-related genes and leads to lipid and protein oxidative by-products. Mutation-associated decreases in SAH and SAMe could compromise needed cysteine availability to generate glutathione during oxidative stress. Hence keratin mutations may prime hepatocytes to oxidative injury, which provides a new potential mechanism for how keratin mutations may predispose patients to cirrhosis.

Keratin mutation primes mouse liver to oxidative injury. Publishing Authors By Initials

For similar biochemical phenomena, metabolism, and nutrition: metabolism: energy metabolism: oxidation-reduction research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: energy metabolism: oxidation-reduction research

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Keratin mutation primes mouse liver to oxidative injury. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Hepatology (Baltimore, Md.)

VOLUME: 41

Page Numbers: 517-25

Journal Abbreviation: Hepatology

ISSN: 0270-9139

DAY: 7

MONTH: Mar

YEAR: 2005

Keratin mutation primes mouse liver to oxidative injury. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8302946

Keratin mutation primes mouse liver to oxidative injury. Keywords Mesh Terms:

KEYWORDS: Oxidation-Reduction

MESH TERMS: etiology

Chemical & Substance for Abstract: Keratin mutation primes mouse liver to oxidative injury. Information

Substance Name: Glutathione

Registry Number: 70-18-8

Grant and Affiliation Information for Keratin mutation primes mouse liver to oxidative injury.

AFFILIATION: Department of Medicine, Palo Alto Veterans Affairs Medical Center and Stanford University Digestive Disease Center, Palo Alto, CA, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01 DK052951-09

ACRONYM: DK

MEDLINETA: Hepatology

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