Kaempferol induces apoptosis in glioblastoma cells through oxidative stress.

Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Research Abstract Details 

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Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Abstract Text:

Vivek Sharma,Christy Joseph,Soumya Ghosh,Anindita Agarwal,Manoj Kumar Mishra,Ellora Sen,

Despite recent advances in understanding molecular mechanisms involved in glioblastoma progression, the prognosis of the most malignant brain tumor continues to be dismal. Because the flavonoid kaempferol is known to suppress growth of a number of human malignancies, we investigated the effect of kaempferol on human glioblastoma cells. Kaempferol induced apoptosis in glioma cells by elevating intracellular oxidative stress. Heightened oxidative stress was characterized by an increased generation of reactive oxygen species (ROS) accompanied by a decrease in oxidant-scavenging agents such as superoxide dismutase (SOD-1) and thioredoxin (TRX-1). Knockdown of SOD-1 and TRX-1 expression by small interfering RNA (siRNA) increased ROS generation and sensitivity of glioma cells to kaempferol-induced apoptosis. Signs of apoptosis included decreased expression of Bcl-2 and altered mitochondrial membrane potential with elevated active caspase-3 and cleaved poly(ADP-ribose) polymerase expression. Plasma membrane potential and membrane fluidity were altered in kaempferol-treated cells. Kaempferol suppressed the expression of proinflammatory cytokine interleukin-6 and chemokines interleukin-8, monocyte chemoattractant protein-1, and regulated on activation, normal T-cell expressed and secreted. Kaempferol inhibited glioma cell migration in a ROS-dependent manner. Importantly, kaempferol potentiated the toxic effect of chemotherapeutic agent doxorubicin by amplifying ROS toxicity and decreasing the efflux of doxorubicin. Because the toxic effect of both kaempferol and doxorubicin was amplified when used in combination, this study raises the possibility of combinatorial therapy whose basis constitutes enhancing redox perturbation as a strategy to kill glioma cells.

Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Publishing Authors By Initials

V Sharma,C Joseph,S Ghosh,A Agarwal,MK Mishra,E Sen,

For similar proteins: thioredoxins research abstracts see: proteins: thioredoxins research

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Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular cancer therapeutics

VOLUME: 6

Page Numbers: 2544-53

Journal Abbreviation: Mol. Cancer Ther.

ISSN: 1535-7163

DAY: 27

MONTH: Sep

YEAR: 2007

Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Information

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LANGUAGE: eng

NlmUniqueID: 101132535

Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Keywords Mesh Terms:

KEYWORDS: Thioredoxins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Information

Substance Name: Caspases

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Kaempferol induces apoptosis in glioblastoma cells through oxidative stress.

AFFILIATION: National Brain Research Centre, near NSG Campus, Manesar, Gurgaon, Haryana-122050, India.

Country: United States

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MEDLINETA: Mol Cancer Ther

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