Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis.

Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis. Research Abstract Details 

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Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis. Abstract Text:

Bridgette D Furman,Jens Strand,W Chad Hembree,Benjamin D Ward,Farshid Guilak,Steven A Olson,

Posttraumatic arthritis is one of the most frequent causes of disability following joint trauma. The objective of this study was to develop a model of a closed articular fracture in the mouse knee joint to quantify the temporal sequence of joint degeneration in a model of posttraumatic arthritis. Closed intraarticular fractures were created in the tibial plateau of adult mice (C57BL/6) using a computer-controlled materials testing system and a custom-built indenter tip. Tibial plateau fractures were classified and imaged over time using high-resolution digital radiography. Animals were sacrificed at 2, 4, 8, and 50 weeks following fracture, and the experimental and contralateral control limbs were harvested for histology and micro-computed tomography (microCT) analysis. By radiographic analysis, tibial plateau fractures closely resembled clinical fractures. More complex and comminuted fractures correlated to significantly higher fracture energies. Histologic analysis demonstrated progressive joint degeneration as measured by a modified Mankin scale, with fibrillation and loss of proteoglycan in the articular cartilage. Subchondral bone thickening was also observed in experimental joints. The induction of a closed intraarticular fracture of the mouse tibial plateau generated a reproducible and clinically relevant joint injury that progressed to osteoarthritis-like changes by histologic and microCT evaluations. The ability to induce joint degeneration without an osteotomy or open arthrotomy provides a valuable new model for studying the natural sequelae of posttraumatic arthritis. Notably, the use of a murine model will facilitate the use of genetically modified animals for the investigation of specific genes implicated in the pathology of posttraumatic arthritis.

Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis. Publishing Authors By Initials

BD Furman,J Strand,WC Hembree,BD Ward,F Guilak,SA Olson,

For similar disorders of environmental origin: wounds and injuries: fractures, bone: tibial fractures research abstracts see: disorders of environmental origin: wounds and injuries: fractures, bone: tibial fractures research

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Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of orthopaedic research : official publica

VOLUME: 25

Page Numbers: 578-92

Journal Abbreviation:

ISSN: 0736-0266

DAY: 3

MONTH: May

YEAR: 2007

Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis. Information

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LANGUAGE: eng

NlmUniqueID: 8404726

Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis. Keywords Mesh Terms:

KEYWORDS: Tibial Fractures

MESH TERMS: radiography

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Grant and Affiliation Information for Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis.

AFFILIATION: Division of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, Box 3389, Durham, North Carolina 27710, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: AR50245

ACRONYM: AR

MEDLINETA: J Orthop Res

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