Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization.

Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. Research Abstract Details 

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Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. Abstract Text:

Y Suzuki,E Itagaki,H Mori,T Hosoya,

The testosterone-binding globulin (TeBG) from bovine serum was purified by affinity chromatography and hydroxylapatite chromatography. The affinity column used was prepared by coupling 17 alpha-carboxyethynyl-17-hydroxy-4-androsten-3-one to aminoethyl-Sepharose. The compound was replaceable by 17alpha-carboxyethynyl-17-hydroxy-5alpha-androstan-3-one, but not by testosterone 17-hemisuccinate, estradiol 17-hemisuccinate, or testosterone 3-(O-carboxymethyl)oxime. The TeBG isolated was homogeneous on analytical polyacrylamide gel electrophoresis and equilibrium centrifugation. The protein was a glycoprotein having a molecular weight of 89,500 and a carbohydrate content of 17%. The association constant (M-1) at 4 degrees C was 1.1 X 10(8) and the number of binding sites per molecule was 0.8. Treatment with guanidine-HCl dissociated the protein into subunits having a molecular weight of 28,400 (about one-third of that of the original molecule). SDS-gel electrophoresis showed that two of the three subunits were slightly larger than the other. The dissociation into subunits could also be accomplished by GEDTA treatment with concomitant loss of testosterone-binding activity. The activity and molecular size were reversibly restored by incubation with excess Ca2+.

Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. Publishing Authors By Initials

Y Suzuki,E Itagaki,H Mori,T Hosoya,

For similar polycyclic compounds: steroids: androstanes: androstenes: androstenols: testosterone research abstracts see: polycyclic compounds: steroids: androstanes: androstenes: androstenols: testosterone research

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Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 1721-31

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1977

Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. Keywords Mesh Terms:

KEYWORDS: Testosterone

MESH TERMS: metabolism

Chemical & Substance for Abstract: Isolation of testosterone-binding globulin from bovine serum by affinity chromatography and its molecular characterization. Information

Substance Name: Edetic Acid

Registry Number: 60-00-4

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Country: JAPAN

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MEDLINETA: J Biochem

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