Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A.

Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. Research Abstract Details 

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Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. Abstract Text:

Papain digestion of porcine IgG in the absence of cysteine resulted in a rather poor yield of fragments (less than 5 per cent). In the presence of cysteine, 70 to 80 per cent of the IgG was degradated in 4 h. Fragments with molecular weight of about 100,000 and 50,000 were separated by gel filtration. The minor fraction (mol. wt. 100,000) most probably consisted of F(c)2 fragments. Fab/c fragments with both Fc and Fab determinants, and also probably some F(ab)2-like fragments. The F(c)2 fragments appeared to be a dimer of Fc stabilized by disulphide bonds. The second main fraction (mol. wt. 50,000) contained Fc and Fab fragments. Mild reduction of the Fc fragments resulted in Fc subfragments of different sizes, thus indicating that papain cleavages had occurred on different spots in the Fc chain. Non-reduced Fc fragments therefore seem to consist of several Fc subfragments stabilized by disulphide bonds. The protein A reactivity of the isolated Fc fragments were rather low compared to the reactivity of intact IgG, respectively 5--15 and 90 per cent. In addition, protein A reactive Fab fragments were isolated from normal porcine IgG.

Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. Publishing Authors By Initials

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

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Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta pathologica et microbiologica Scandinavica. S

VOLUME: 87C

Page Numbers: 177-83

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ISSN: 0304-1328

DAY: 26

MONTH: Jun

YEAR: 1979

Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. Information

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LANGUAGE: eng

NlmUniqueID: 7508469

Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: immunology

Chemical & Substance for Abstract: Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. Information

Substance Name: Papain

Registry Number: EC 3.4.22.2

Grant and Affiliation Information for Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A.

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Country: DENMARK

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MEDLINETA: Acta Pathol Microbiol Scand [C

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