Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +.

Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +. Research Abstract Details 

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Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +. Abstract Text:

H Uchida,Y X Chen,H Morinaga,Y Hayashi,A Matsuda,T Obata,Y Endo,T Sasaki,

Deoxycytidine kinase from Ehrlich carcinoma cells was purified 10400-fold by ammonium sulfate fractionation and affinity chromatography using Sepharose 4B coupled to 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine , with a yield of 45%. The purified enzyme preparation showed a single major band with a molecular weight of 32000 on SDS-PAGE. The enzyme phosphorylated deoxyadenosine, deoxyguanosine, cytidine, and several deoxycytidine analogues as well as deoxycytidine. Also, the kinetic parameters of the enzyme for the substrates were estimated.

Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +. Publishing Authors By Initials

H Uchida,YX Chen,H Morinaga,Y Hayashi,A Matsuda,T Obata,Y Endo,T Sasaki,

For similar chemical and pharmacologic phenomena: kinetics research abstracts see: chemical and pharmacologic phenomena: kinetics research

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Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biological & pharmaceutical bulletin

VOLUME: 22

Page Numbers: 83-6

Journal Abbreviation: Biol. Pharm. Bull.

ISSN: 0918-6158

DAY: 20

MONTH: Jan

YEAR: 1999

Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +. Information

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LANGUAGE: eng

NlmUniqueID: 9311984

Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +. Keywords Mesh Terms:

KEYWORDS: Kinetics

MESH TERMS: metabolism

Chemical & Substance for Abstract: Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +. Information

Substance Name: Deoxycytidine Kinase

Registry Number: EC 2.7.1.74

Grant and Affiliation Information for Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine++ +.

AFFILIATION: Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan.

Country: JAPAN

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MEDLINETA: Biol Pharm Bull

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