Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride.

Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride. Research Abstract Details 

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Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride. Abstract Text:

M Ono,M Ando,T Shimada,K Furuno,K Kato,M Kuwano,

Chloroquine-resistant (CQr) clones (CQ-21 and CQ-22) have been isolated from mutagenized hamster lung V79 cells by exposing the cells to a high dose of chloroquine. CQ-21 and CQ-22 showed about 3-fold higher resistance to chloroquine than the parental V79 cells, and they showed specific cross-resistance to another amine, NH4Cl, which is also concentrated in lysosomes. CQr clone showed no cross-resistance to other unrelated agents. Chloroquine-induced inhibition of [125I]ricin internalization was observed in both cell lines at neutral pH, but the inhibition of uptake was less in the variant. Also, the degradation of endogenous protein was slowed in the mutant; further, treatment of cells with 30 micrograms/ml of chloroquine inhibited the degradation of endogenous proteins in the parental V79, but not in CQ-22 cells. Similar levels of acid phosphatase, beta-glucuronidase and cathepsin D were observed in V79 and CQ-22 cells, but the level of cathepsin B was lower in the mutant. Electron microscopy showed an increased number of electron-dense bodies, possibly autophagosomes/lysosomes, in the mutant cells grown for 4 days with 5 micrograms/ml of chloroquine. Similar aberrant structures were observed in the parental V79 cells treated for only 3 h with 5 micrograms/ml of chloroquine.

Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride. Publishing Authors By Initials

M Ono,M Ando,T Shimada,K Furuno,K Kato,M Kuwano,

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Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 1493-503

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1983

Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride. Information

Substance Name: Chloroquine

Registry Number: 54-05-7

Grant and Affiliation Information for Isolation of chloroquine-resistant Chinese hamster V79 cell variants that are also resistant to ammonium chloride.

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Country: JAPAN

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MEDLINETA: J Biochem

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