Isolation of chlorophylls a and b from spinach by counter-current chromatography.

Isolation of chlorophylls a and b from spinach by counter-current chromatography. Research Abstract Details 

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Isolation of chlorophylls a and b from spinach by counter-current chromatography. Abstract Text:

Carole Jubert,George Bailey,

A method for the isolation of chlorophylls from spinach by counter-current chromatography was developed. An initial extraction protocol was devised to avoid the notorious sensitivity of chlorophylls to degradation by light, heat, oxygen, acids and bases. Further purification and separation of chlorophylls a and b were achieved using counter-current chromatography. Chlorophyll structures and purities were established by HPLC, fast atom bombardment mass spectrometry and nuclear magnetic resonance. Purity was estimated to be >95% (100% by HPLC). Typical yields from 30g of freeze-dried spinach were 300mg of chlorophyll a and 100mg of chlorophyll b.

Isolation of chlorophylls a and b from spinach by counter-current chromatography. Publishing Authors By Initials

C Jubert,G Bailey,

For similar plants: plant families and groups: angiosperms: chenopodiaceae: spinacia oleracea research abstracts see: plants: plant families and groups: angiosperms: chenopodiaceae: spinacia oleracea research

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MEDLINE DATE:

Isolation of chlorophylls a and b from spinach by counter-current chromatography. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of chromatography. A

VOLUME: 1140

Page Numbers: 95-100

Journal Abbreviation:

ISSN: 0021-9673

DAY: 11

MONTH: 12

YEAR: 2006

Isolation of chlorophylls a and b from spinach by counter-current chromatography. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9318488

Isolation of chlorophylls a and b from spinach by counter-current chromatography. Keywords Mesh Terms:

KEYWORDS: Spinacia oleracea

MESH TERMS: chemistry

Chemical & Substance for Abstract: Isolation of chlorophylls a and b from spinach by counter-current chromatography. Information

Substance Name: chlorophyll b

Registry Number: 519-62-0

Grant and Affiliation Information for Isolation of chlorophylls a and b from spinach by counter-current chromatography.

AFFILIATION: Linus Pauling Institute, Oregon State University, 435 Weniger Hall, Corvallis, OR 97331, USA. carole.jubert@oregonstate.edu

Country: Netherlands

AGENCY: United States NCI

GRANT: P01 CA90890

ACRONYM: CA

MEDLINETA: J Chromatogr A

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