Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein.

Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein. Research Abstract Details 

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Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein. Abstract Text:

M Nonaka,M Takahashi,S Natsuume-Sakai,M Nonaka,S Tanaka,A Shimizu,T Honjo,

cDNA clones specific for the fourth component of mouse complement (C4) and its hormonally regulated isotype, sex-linked protein (Slp), were isolated using as a probe a 20-mer synthetic oligonucleotide corresponding to a known sequence of human C4 cDNA. Two types of clones, one specific for C4 (pFC4/10, with a 3.7 kilobase insert) and one specific for Slp (pFSlp/1, with a 4.7 kilobase insert), were isolated from liver cDNA libraries constructed from the Slp-producing FM mouse strain. The cDNA inserts of these clones shared 70% of the restriction sites determined. Only one type of clone was isolated from the Slp-negative DBA/1 strain; this type showed restriction maps indistinguishable from that of pFC4/10. pFC4/10 and pFSlp/1 displayed extensive homology: 94% nucleotide homology and 89% derived amino acid homology in the C4a region and 92% nucleotide homology and 89% derived amino acid homology in the thiol-ester region. An Arg-Gln-Lys-Arg sequence in the beta-alpha junction and a Cys-Ala-Glu-Gln sequence in the thiol-ester site were identified for both proteins. A remarkable divergency between C4 and Slp sequences was recognized in the region immediately following the C4a sequence.

Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein. Publishing Authors By Initials

M Nonaka,M Takahashi,S Natsuume-Sakai,M Nonaka,S Tanaka,A Shimizu,T Honjo,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research

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Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 81

Page Numbers: 6822-6

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 11

MONTH: Nov

YEAR: 1984

Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein. Keywords Mesh Terms:

KEYWORDS: RNA, Messenger

MESH TERMS: analysis

Chemical & Substance for Abstract: Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein.

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Country: UNITED STATES

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MEDLINETA: Proc Natl Acad Sci U S A

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ACCESSION NUMBER: M12973

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