Isolation murine mesenchymal stem cells by positive selection.

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Isolation murine mesenchymal stem cells by positive selection. Abstract Text:

Samad Nadri,Masoud Soleimani,Samad Nadri,Masoud Soleimani,Samad Nadri,Masoud Soleimani,

Isolation and purification of mesenchymal stem cells (MSCs) from mouse via plastic adherent cultures is arduous because of the unwanted growth of hematopoietic cells and non-MSCs. In this work, homogenous populations of CD34(+) MSCs from mouse bone marrow were isolated via positive selection. For this purpose, C57Bl/6 mice were killed and bone marrow cells were aspirated before incubation with magnetic bead conjugated to anti-CD34 antibody. A sample of positively selected CD34(+) cells were prepared for flow cytometry to examine the expression of CD34 antigen and others were subcultured in a 25-cm(2) culture flask. To investigate the mesenchymal nature, the plastic adherent cultivated cells were induced to differentiate along osteoblastic and adipogenic lineages. Furthermore, the expression of some surface markers was investigated by flow cytometry. According to the result, purified populations of fibroblast-like CD34(+) cells were achieved in the first passage (1 wk after culture initiation). The cells expressed CD34, CD44, Sca-1, and Vcam-1 antigens (markers) but not CD11b and CD45. They were capable of differentiating into osteocytes and adipocytes. This study indicated that our protocol can result in the efficient isolation of homogenous populations of MSCs from C57BL/6 mouse bone marrow. We have shown that murine bone marrow-derived CD34(+) cells with plastic adherent properties and capability of differentiating into skeletal lineages in vitro are MSCs.

Isolation murine mesenchymal stem cells by positive selection. Publishing Authors By Initials

S Nadri,M Soleimani,S Nadri,M Soleimani,S Nadri,M Soleimani,

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MEDLINE DATE: 2007 Sep-Oct

Isolation murine mesenchymal stem cells by positive selection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: In vitro cellular & developmental biology. Animal

VOLUME: 43

Page Numbers: 276-82

Journal Abbreviation: In Vitro Cell. Dev. Biol. Anim

ISSN: 1071-2690

DAY: 12

MONTH: 09

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 9418515

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Grant and Affiliation Information for Isolation murine mesenchymal stem cells by positive selection.

AFFILIATION: Stem Cells Department, Stem Cell Technology, Co, Tehran, Iran.

Country: United States

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MEDLINETA: In Vitro Cell Dev Biol Anim

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