Isolation from cochlea of a novel human intronless gene with predominant fetal expression.

Isolation from cochlea of a novel human intronless gene with predominant fetal expression. Research Abstract Details 

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Isolation from cochlea of a novel human intronless gene with predominant fetal expression. Abstract Text:

We have cloned a novel human gene, designated PFET1 (predominantly fetal expressed T1 domain) (HUGO-approved symbol KCTD12 or C13orf2), by subtractive hybridization and differential screening of human fetal cochlear cDNA clones. Also, we have identified the mouse homolog, designated Pfet1. PFET1/Pfet1 encode a single transcript of approximately 6 kb in human, and three transcripts of approximately 4, 4.5, and 6 kb in mouse with a 70% GC-rich open reading frame (ORF) consisting of 978 bp in human and 984 bp in mouse. Both genes have unusually long 3' untranslated (3' UTR) regions (4996 bp in human PFET1, 3700 bp in mouse Pfet1) containing 12 and 5 putative polyadenylation consensus sequences, respectively. Pfetin, the protein encoded by PFET1/Pfet1, is predicted to have 325 amino acids in human and 327 amino acids in mouse and to contain a voltage-gated potassium (K+) channel tetramerization (T1) domain. Otherwise, to date these genes have no significant homology to any known gene. PFET1 maps to the long arm of human chromosome 13, in band q21 as shown by FISH analysis and STS mapping. Pfet1 maps to mouse chromosome 14 near the markers D14Mit8, D14Mit93, and D14Mit145.1. The human 6 kb transcript is present in a variety of fetal organs, with highest expression levels in the cochlea and brain and, in stark contrast, is detected only at extremely low levels in adult organs, such as brain and lung. Immunohistochemistry with a polyclonal antibody raised against a synthetic peptide to PFET1 sequence (pfetin) reveals immunostaining in a variety of cell types in human, monkey, mouse, and guinea pig cochleas and the vestibular system, including type I vestibular hair cells.

Isolation from cochlea of a novel human intronless gene with predominant fetal expression. Publishing Authors By Initials

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Isolation from cochlea of a novel human intronless gene with predominant fetal expression. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the Association for Research in Otolary

VOLUME: 5

Page Numbers: 185-202

Journal Abbreviation: J. Assoc. Res. Otolaryngol.

ISSN: 1525-3961

DAY: 17

MONTH: Jun

YEAR: 2004

Isolation from cochlea of a novel human intronless gene with predominant fetal expression. Information

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LANGUAGE: eng

NlmUniqueID: 100892857

Isolation from cochlea of a novel human intronless gene with predominant fetal expression. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Isolation from cochlea of a novel human intronless gene with predominant fetal expression. Information

Substance Name: pfetin protein, mouse

Registry Number: 0

Grant and Affiliation Information for Isolation from cochlea of a novel human intronless gene with predominant fetal expression.

AFFILIATION: Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NIDCD

GRANT: T32DC0019

ACRONYM: DC

MEDLINETA: J Assoc Res Otolaryngol

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