Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans.

Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans. Research Abstract Details 

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Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans. Abstract Text:

K C Hazen,J E Cutler,

The human pathogenic, dimorphic fungus Candida albicans produces in vitro a factor designated morphogenic autoregulatory substance (MARS) which suppresses yeast to mycelial transition. MARS is best produced when a high concentration of yeast cells (2 X 10(8)/ml) in a chemically defined medium is incubated at 37 degrees C for 9-15 h. MARS was isolated to apparent homogeneity by the following successive steps: charcoal absorption; pyridine elution; ether extraction; high pressure liquid chromatography, and Sephadex LH-20 chromatography. A 469-fold increase in purity was obtained by these procedures. The use of purified MARS to study C. albicans yeast to mycelial transition may lead to a better understanding of morphogeneis of this important opportunistic pathogen.

Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans. Publishing Authors By Initials

KC Hazen,JE Cutler,

For similar chemical and pharmacologic phenomena: kinetics research abstracts see: chemical and pharmacologic phenomena: kinetics research

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Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 777-83

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1983

Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans. Keywords Mesh Terms:

KEYWORDS: Kinetics

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans. Information

Substance Name: Growth Substances

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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