Isolation and purification of functional bovine lung mast cells (BLMCs).

Isolation and purification of functional bovine lung mast cells (BLMCs). Research Abstract Details 

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Isolation and purification of functional bovine lung mast cells (BLMCs). Abstract Text:

J Stahl,E Cook,S Dong,R Saban,F M Graziano,

Purified pulmonary mast cells were obtained from bovine lung using a combination of enzymatic digestion of tissue, density gradient centrifugation using Percoll, and centrifugal elutriation. In the initial procedure, lung tissue was enzymatically digested with collagenase, hyaluronidase, protease and elastase in three 30 min incubations at 37 degrees C. Monodispersed cell suspensions contained between 2 and 6% mast cells. Further purification of these mast cells by Percoll gradients and elutriation consistently yielded mast cells of > 90% purity. These cells were morphologically intact, viable and functional, as determined by histamine release evoked by secretagogue challenge. Incubation of BLMCs with Pasteurella haemolytica A1 culture supernate containing leucotoxin (LCT) alone, resulted in increased histamine release compared to controls. LCT also potentiated calcium ionophore (CaI)-induced histamine release from BLMCs.

Isolation and purification of functional bovine lung mast cells (BLMCs). Publishing Authors By Initials

J Stahl,E Cook,S Dong,R Saban,FM Graziano,

For similar organic chemicals: amines: ethylamines: phenethylamines: p-methoxy-n-methylphenethylamine research abstracts see: organic chemicals: amines: ethylamines: phenethylamines: p-methoxy-n-methylphenethylamine research

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Isolation and purification of functional bovine lung mast cells (BLMCs). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

VOLUME: 43

Page Numbers: 45-53

Journal Abbreviation: Zentralblatt Veterinarmedizin

ISSN: 0514-7166

DAY: 13

MONTH: Mar

YEAR: 1996

Isolation and purification of functional bovine lung mast cells (BLMCs). Information

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LANGUAGE: eng

NlmUniqueID: 331325

Isolation and purification of functional bovine lung mast cells (BLMCs). Keywords Mesh Terms:

KEYWORDS: p-Methoxy-N-methylphenethylamine

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Isolation and purification of functional bovine lung mast cells (BLMCs). Information

Substance Name: Collagenases

Registry Number: EC 3.4.24.-

Grant and Affiliation Information for Isolation and purification of functional bovine lung mast cells (BLMCs).

AFFILIATION: Department of Medicine, University of Wisconsin Medical School, Madison 53792-3244, USA.

Country: GERMANY

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MEDLINETA: Zentralbl Veterinarmed B

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