Isolation and purification of a low-molecular-weight skeletal growth factor from human bones.

Isolation and purification of a low-molecular-weight skeletal growth factor from human bones. Research Abstract Details 

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Isolation and purification of a low-molecular-weight skeletal growth factor from human bones. Abstract Text:

S Mohan,J C Jennings,T A Linkhart,D J Baylink,

A low-molecular-weight potent bone cell mitogen termed human skeletal growth factor (human SGF) was purified to homogeneity from human bone matrix. Extraction and initial purification steps were done under dissociative conditions to separate human SGF from high-molecular-weight complexes of bone matrix proteins. SGF activity was extracted from human femoral heads by demineralization with 10% EDTA in the presence of 4 M guanidine-HCl and proteinase inhibitors and was purified by hydroxyapatite, HPLC gel-filtration and HPLC reverse-phase chromatography. Human SGF thus purified was homogeneous by HPLC reverse-phase chromatography and SDS-polyacrylamide gel electrophoresis. The relative molecular mass of human SGF purified under dissociative conditions was 11,000. Human SGF stimulated bone cell proliferation ([3H]thymidine incorporation and cell number) at picomolar concentrations, with half maximum activity at 2-3 ng/ml (180-270 pM). Human SGF constitutes 0.00024% of organic bone matrix by weight.

Isolation and purification of a low-molecular-weight skeletal growth factor from human bones. Publishing Authors By Initials

S Mohan,JC Jennings,TA Linkhart,DJ Baylink,

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Isolation and purification of a low-molecular-weight skeletal growth factor from human bones. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biochimica et biophysica acta

VOLUME: 884

Page Numbers: 234-42

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 19

MONTH: Nov

YEAR: 1986

Isolation and purification of a low-molecular-weight skeletal growth factor from human bones. Information

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LANGUAGE: eng

NlmUniqueID: 217513

Isolation and purification of a low-molecular-weight skeletal growth factor from human bones. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Isolation and purification of a low-molecular-weight skeletal growth factor from human bones. Information

Substance Name: Proteins

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Grant and Affiliation Information for Isolation and purification of a low-molecular-weight skeletal growth factor from human bones.

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Country: NETHERLANDS

AGENCY: United States NIADDK

GRANT: AM31062

ACRONYM: AM

MEDLINETA: Biochim Biophys Acta

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