Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes.

Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes. Research Abstract Details 

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Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes. Abstract Text:

S Hamada,N Okahashi,T Yamamoto,I Morisaki,S M Michalek,J R McGhee,

Group A carbohydrate antigens were prepared from isolated cell walls or whole cells of Streptococcus pyogenes strain Sv (group A, M type 3). Cell walls were lysate by the enzymatic action of M1 endo-N-acetylmuramidase. The cell wall lysate was concentrated and chromatographed on a Sephadex G-100 column. Two fractions reactive with group A antisera were obtained. These were composed of N-acetylglucosamine, rhamnose, and peptidoglycan components such as glutamic acid, lysine, alanine, N-acetylmuramic acid and N-acetylglucosamine. For comparison, hot TCA extract of the cell walls (TCAgA) or Rantz and Randall extract of the whole cells (RRgA) was purified chromatographically. The latter two also contained rhamnose and glucosamine, and were reactive with group A antisera. The molecular weights of M1gA antigens were larger than those of TCAgA and RRgA. Hapten inhibition study indicated that N-acetylglucosamine was the most potent inhibitor.

Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes. Publishing Authors By Initials

S Hamada,N Okahashi,T Yamamoto,I Morisaki,SM Michalek,JR McGhee,

For similar bacteria: gram-positive bacteria: gram-positive cocci: streptococcaceae: streptococcus: streptococcus pyogenes research abstracts see: bacteria: gram-positive bacteria: gram-positive cocci: streptococcaceae: streptococcus: streptococcus pyogenes research

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Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zentralblatt für Bakteriologie, Mikrobiologie, und

VOLUME: 256

Page Numbers: 37-48

Journal Abbreviation:

ISSN: 0176-6724

DAY: 12

MONTH: Nov

YEAR: 1983

Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8403032

Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes. Keywords Mesh Terms:

KEYWORDS: Streptococcus pyogenes

MESH TERMS: immunology

Chemical & Substance for Abstract: Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes. Information

Substance Name: Haptens

Registry Number: 0

Grant and Affiliation Information for Isolation and immunochemical characterization of carbohydrate antigens prepared from group A Streptococcus pyogenes.

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Country: GERMANY, WEST

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MEDLINETA: Zentralbl Bakteriol Mikrobiol

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