[Isolation and culture of tumor stem cells from human brain glioma tissues]

[Isolation and culture of tumor stem cells from human brain glioma tissues] Research Abstract Details 

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[Isolation and culture of tumor stem cells from human brain glioma tissues] Abstract Text:

Qiang Huang,Jun Dong,Yu-de Zhu,Quan-bin Zhang,Xiao-yan Ji,Ai-dong Wang,Qing Lan,Qiang Huang,Jun Dong,Yu-de Zhu,Quan-bin Zhang,Xiao-yan Ji,Ai-dong Wang,Qing Lan,

OBJECTIVE: To isolate and culture tumor stem cells from glioma tissues obtained at surgical operation and to study their biological characteristics. METHODS: Glioma tissues obtained from surgically resected specimens of 8 patients were fully chopped, trypsinized, and filtered to prepare single cell suspensions. The cells were cultured in serum-free medium with EGF, LIF and bFGF. CD133(+) cells were purified by magnetic cell sorting, and cultured continuously in vitro to obtain tumor cell spheres. Tumor stem cells of the 5th passage were induced to differentiate with 10% FBS, and expression of cell differentiation markers such as Nestin, MAP2, GFAP was evaluated with immunocytochemistry techniques. RESULTS: CD133(+) cells were successfully separated and cultured from one anasplastic mixed astrocyte-ependymocyte type glioma specimen. These cells maintained a sphere-like growth status in vitro (3 months, 14 passages), and can self-renew, proliferate and conditionally differentiate into MAP2(+) and GFAP(+) cells. However, CD133(-) cells did not possess these properties. CONCLUSION: Glioma tissue contains tumor stem cells. Those cells can be cultured and passaged in vitro for a long term, and therefore to offer new approaches for studying cellular and molecular biology of glioma.

[Isolation and culture of tumor stem cells from human brain glioma tissues] Publishing Authors By Initials

Q Huang,J Dong,YD Zhu,QB Zhang,XY Ji,AD Wang,Q Lan,Q Huang,J Dong,YD Zhu,QB Zhang,XY Ji,AD Wang,Q Lan,

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[Isolation and culture of tumor stem cells from human brain glioma tissues] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua zhong liu za zhi [Chinese journal of onco

VOLUME: 28

Page Numbers: 331-3

Journal Abbreviation: Zhonghua Zhong Liu Za Zhi

ISSN: 0253-3766

DAY: 18

MONTH: May

YEAR: 2006

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LANGUAGE: chi

NlmUniqueID: 7910681

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AFFILIATION: Neurosurgery Department and Brain Tumor Research Laboratory, 2nd Affiliated Hospital of Suzhou University, Suzhou 215004, China. szqhdnah@pub.sz.jsinfo.net

Country: China

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MEDLINETA: Zhonghua Zhong Liu Za Zhi

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