[Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord]

[Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord] Abstract Text:

Sai-Li Fu,Zheng-Wen Ma,Lan Yin,Pei-Hua Lu,Xiao-Ming Xu,

The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells (NSCs) from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used. The results are as follows. (1) In the presence of fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF), both brain- and spinal cord-derived stem cells proliferated and expanded in vitro for 8 - 10 passages (over 60 d). The period of expansion resulted in a 10(6)-fold increase in brain-derived NSCs and 10(5)-fold increase in spinal cord-derived NSCs. These proliferating cells expressed nestin. (2) In the medium containing 1% FBS, the two NSCs populations could be induced to differentiate into neurons, astrocytes and oligodentrocytes. The percentage of neurons (beta-tubulin III-ir) differentiated from brain-derived NSCs decreased rapidly from 11.95+/-2.5% at passage 2 (P(2)) to 1.97+/-1.16% at passage 5 (P5). Significant difference was shown between P(2) and P(5) (P<0.01). The percentage of oligodentrocytes (Rip-ir) differentiated from brain-derived NSCs remained mostly unchanged from 8.66+/-2.93% at P(2) to 9.12+/-1.13% at P(5). The same differentiation patterns were found in spinal cord-derived NSCs. All these results indicate that both embryonic rat brain- and spinal cord-derived NSCs can expand and proliferate in vitro through multiple passages, and retain the capacity to differentiate into all three major types of cells in the central nervous system.

[Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord] Publishing Authors By Initials

SL Fu,ZW Ma,L Yin,PH Lu,XM Xu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Sheng li xue bao : [Acta physiologica Sinica]

VOLUME: 55

Page Numbers: 278-83

Journal Abbreviation:

ISSN: 0371-0874

DAY: 25

MONTH: Jun

YEAR: 2003

[Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 20730130

[Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord]

AFFILIATION: Department of Neurobiology, Shanghai Second Medical University, Shanghai 200025.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Sheng Li Xue Bao

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord Related Publications

• Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
• Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography.
• Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat.
• [Analysis of the mutations of BRCA1 in 9 familiar breast cancer patients]
• [Application of the interaction models between the polymorphism(s) of metabolic gene(s) and environmental exposure]
• [Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-kappaB]
• [Changes in the activity of platelet L-arginine/nitric oxide pathway in hypercholesterolemia patients]
• [Clinical significance of changes in insulin resistance and secretion in multiple organ dysfunction syndrome patients complicated by trauma and hemorrhage]
• [Cloning and sequencing of the genes coding for the histidine-rich protein II of Plasmodium falciparum]
• [Contrast agents in magnetic resonance imaging: development and problems]
• [Differentially expressed genes associated with cold acclimation]
• [Effects of embryonic neural stem cells and glial cell line-derived neurotrophic factor in the repair of spinal cord injury]
• [Effects of ulinastatin on interleukin-8 during one-lung ventilation in surgery]
• [Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord]
• [Mechanism of modulation of synaptic efficacy by stress and learning]
• [Opinion on hydatidosis control by immuno-prophylaxis]
• [Oral mucosa graft combined with a local turn-over flap for urethral reconstruction in various hypospadias]
• [Pharmacognostical studies on the roots of Euphorbia prolifera]
• [Pharmacological comparison between virosecurinine and securinine]
• [Phenotyping and genotyping studies in a family with the compound heterozygosity for a deletional delta beta-thalassemia and a beta-thalassemia]
• [Properties of whole-cell potassium currents in mechanically dissociated Drosophila larval central neurons]
• [Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot]
• [Retrospective analysis of effectiveness of tonsillar squamous cell carcinoma: report of 108 cases]
• [Significance of hRad17 mRNA expression in human gastric cancer]
• [Study of functional L1 retrotransposon in human type 2 diabetes susceptibility loci]
• [Study on diarrhea disease and Escherichia coli strains harboring HPI pathogenicity island of Yersinia enterolitica in Shandong province]
• [The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice]
• [The influence of postburn administration of rhGH on the host inflammatory response]
• [The transplantation of latissimus dorsi flap of the base of T shape artery with the pedicle of the thoracodorsal artery]
• [Tissue-specific expression of Na(+)-H(+) exchanger isoforms at two developmental stages of human fetus]