Isolation and compositional analysis of trypanosomatid editosomes.

Isolation and compositional analysis of trypanosomatid editosomes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Isolation and compositional analysis of trypanosomatid editosomes. Abstract Text:

Aswini K Panigrahi,Achim Schnaufer,Kenneth D Stuart,

Most mitochondrial (mt) mRNAs in trypanosomes undergo posttranscriptional RNA editing, which inserts and deletes uridines (Us) to produce the mature and functional mRNA. The editing process is catalyzed by multiple enzymatic steps and is carried out by an approximately 20S macromolecular complex, the editosome. Editosomes have been purified from Trypanosoma brucei using various techniques including combinations of column chromatography, gradient sedimentation, monoclonal antibody affinity, and TAP-tag affinity approaches. This article describes in detail the methods for editosome purification and identification of protein components by mass spectrometry analyses. It also describes the methods for isolation and analysis of TAP-tagged mutagenized complexes.

Isolation and compositional analysis of trypanosomatid editosomes. Publishing Authors By Initials

AK Panigrahi,A Schnaufer,KD Stuart,

For similar animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: kinetoplastida: trypanosomatina: trypanosoma: trypanosoma brucei brucei research abstracts see: animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: kinetoplastida: trypanosomatina: trypanosoma: trypanosoma brucei brucei research

PUBMED ID PMID:

MEDLINE DATE:

Isolation and compositional analysis of trypanosomatid editosomes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Methods in enzymology

VOLUME: 424

Page Numbers: 3-24

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 3

MONTH: 12

YEAR: 2007

Isolation and compositional analysis of trypanosomatid editosomes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 212271

Isolation and compositional analysis of trypanosomatid editosomes. Keywords Mesh Terms:

KEYWORDS: Trypanosoma brucei brucei

MESH TERMS: metabolism

Chemical & Substance for Abstract: Isolation and compositional analysis of trypanosomatid editosomes. Information

Substance Name: RNA-Binding Proteins

Registry Number: 0

Grant and Affiliation Information for Isolation and compositional analysis of trypanosomatid editosomes.

AFFILIATION: Seattle Biomedical Research Institute, Seattle, Washington, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 42188

ACRONYM: GM

MEDLINETA: Methods Enzymol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Isolation and compositional analysis of trypanosomatid editosomes Related Publications

• An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei.
• An improved replica-exchange sampling method: Temperature intervals with global energy reassignment.
• Arm numbness and weakness after recurrent shoulder dislocation: 1028: may 30 3:35 PM - 3:55 PM.
• Book review: Cancer chemotherapy and biological response modifiers, Annual 17.
• Book review: Cancer in the elderly: A nursing and medical perspective.
• Can breast cancer be prevented?
• Comparative sequence analysis and tissue localization of members of the SLC6 family of transporters in adult Drosophila melanogaster.
• Dendritic mechanisms controlling spike-timing-dependent synaptic plasticity.
• Development and validation of the Inventory of Depression and Anxiety Symptoms (IDAS).
• Evidence-based medicine for the new millennium: Critical appraisal and pragmatic approach.
• Historical reflections on the afterhyperpolarization--firing rate relation of vertebrate spinal neurons.
• IGF-1 controls GLUT3 expression in muscle via the transcriptional factor Sp1.
• Interstrain inseminations and embryo transfers between the SLA miniature pig and standard crossbred pig.
• Isolation and compositional analysis of trypanosomatid editosomes.
• On deanships: survival tips for a hardship tour of duty.
• Oxa1 directly interacts with Atp9 and mediates its assembly into the mitochondrial F1Fo-ATP synthase complex.
• Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy.
• Putting beta-diversity on the map: broad-scale congruence and coincidence in the extremes.
• RNA editing in Trypanosoma brucei requires three different editosomes.
• Recommending pathway genes using a compendium of clustering solutions.
• Response of dairy heifers to Prostaglandin F(2alpha) after treatment with human chorionic gonadotropin(1).
• The adolescent.
• The phylogenetic problem of Huia (Amphibia: Ranidae).
• The prevalence of postpartum depression: the relative significance of three social status indices.
• The teaching of the use of the polarizing microscope.
• Uridine insertion/deletion editing activities.
• Variation in incidence of pneumococcal and meningococcal disease across Europe.
• Wormlike aggregates from a supramolecular coordination polymer and a diblock copolymer.
• [Not Available.]
• [Not Available.]