Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance.

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Abstract Text:

Y Yang,Y Z Ma,Z S Xu,X M Chen,Z H He,Z Yu,M Wilkinson,H D Jones,P R Shewry,L Q Xia,Y Yang,Y Z Ma,Z S Xu,X M Chen,Z H He,Z Yu,M Wilkinson,H D Jones,P R Shewry,L Q Xia,

Pre-harvest sprouting (PHS) of wheat reduces the quality and economic value of grain, and increasing PHS tolerance is one of the most important traits in wheat breeding. Two new Vp-1B alleles related to PHS tolerance were identified on the 3BL chromosome of bread wheat and were designated Vp-1Bb and Vp-1Bc. Sequence analysis showed that Vp-1Bb has a 193 bp insertion and Vp-1Bc has a 83 bp deletion located in the third intron region of the Vp-1B gene, and that they shared 95.43% and 97.89% similarity, respectively, with the sequence of AJ400713 (Vp-1Ba) at the nucleotide level. Their sequences were deposited in the GenBank under the accession numbers DQ517493 and DQ517494. Semi-quantitative RT-PCR analysis showed that alternatively spliced transcripts of the Vp-1A, Vp-1B, and Vp-1D homologues were present and there were no differences in the splicing patterns or abundances of Vp-1A and Vp-1D from embryos 35 d after pollination between PHS-tolerant and -susceptible cultivars. Although Vp-1Ba, Vp-1Bb, and Vp-1Bc could each produce a set of transcripts, only one was correctly spliced and had the capacity to encode the full-length VP1 protein and was more highly expressed with Vp-1Bb and Vp-1Bc than with Vp-1Ba. Comparison of the expression patterns of Vp-1Ba, Vp-1Bb, and Vp-1Bc on different days after pollination also revealed that the expression of these genes was developmentally regulated. Furthermore, genotypes with different levels of tolerance to PHS respond differently to ABA exposure and differences in transcript levels of Vp-1Ba, Vp-1Bb, and Vp-1Bc were observed after ABA treatment. The results indicated that insertion or deletion in the third intron region might affect the expression of the Vp-1B gene and its sensitivity to ABA, and thus resistance to PHS.

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Publishing Authors By Initials

Y Yang,YZ Ma,ZS Xu,XM Chen,ZH He,Z Yu,M Wilkinson,HD Jones,PR Shewry,LQ Xia,Y Yang,YZ Ma,ZS Xu,XM Chen,ZH He,Z Yu,M Wilkinson,HD Jones,PR Shewry,LQ Xia,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of experimental botany

VOLUME: 58

Page Numbers: 2863-71

Journal Abbreviation: J. Exp. Bot.

ISSN: 0022-0957

DAY: 13

MONTH: 07

YEAR: 2007

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9882906

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance.

AFFILIATION: Institute of Crop Science/National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), No. 12 Zhongguancun South Street, Beijing 100081, China.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Exp Bot

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance Related Publications

• Treating external chylous fistula with retroperitoneal lymphangiectomy plus lymph-vein shunting.
• [Acute fluoroacetamide poisoning with main damage to the heart]
• [Analysis of season variation of methyleugenol and safrole in Asarum heterotropoides by gas chromatography]
• [Changes in field excitatory postsynaptic potential and population spike in CA1 region of rat hippocampal slices following low-frequency stimulation]
• [Changes of neuropeptide Y mRNA expression in the cerebral tissues of rat models of subarachnoid hemorrhage]
• [Chromosome analysis and phenotype location analysis on a patient with the karyotype of 45, XX, -13/46, XX, r(13)/46, XX, r(13;13)/47, XX, 2r(13)(p13q32.3)]
• [Classification and surgical treatment of intrathoracic esophageal injury caused by foreign body]
• [Clinical investigation in the expression of matrix metalloproteinase 2 mRNA in the hyperplastic scar in burn patients]
• [Clinical study on the changes in plasma sodium level and blood erythrocyte after resuscitation with different fluid regimes at early postburn stage]
• [Effect of iron on vasoconstriction in the isolated rat aorta]
• [Effects of the alkaloid sinomenine on COX-2 activity and acute rejection in heart allografts: an experiment with rats]
• [Efficacy of autologous renal tumor cell lysate-loaded dendritic cell vaccine in combination with cytokine-induced killer cells on advanced renal cell carcinoma--a report of ten cases]
• [Experimental studies on the biocompatibility of spongy PLGA-collagen membrane as a tissue-engineering dermal scaffold]
• [Frequency analysis of autosomal dominant spinocerebellar ataxias in Han population in the Chinese mainland and clinical and molecular characterization of spinocerebellar ataxia type 6]
• [Geometric active contour model with color and intensity priors for medical image segmentation]
• [Influence of microwave ablation and surgical resection of small primary hepatocellular carcinoma on hematogenous dissemination of tumor cells]
• [Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats]
• [Investigation on the association of safety perception and safety behaviors with occupational injuries in steel-workers]
• [Method of cyanogen bromide-fluorimetry determination of trace amount of selenomethionine in grain and blood]
• [Molecular cloning, sequence and function analyses and specific expression of gene BmICAD related to apoptosis in silkworm, Bombyx mori]
• [Off pump coronary artery bypass grafting in 51 cases]
• [Proliferation inhibition effect of indomethacin on CML cells associated with down-regulation of phosphorylated STAT1/STAT5 and inhibition of COX-2 expression.]
• [Rapid development of cosmetic medicine in China]
• [Reform of the pedicled abdominal flap and its clinical application]
• [Relation of genetic polymorphism of NQO1 and GSTT1 with risks of chronic benzene poisoning]
• [Significance of bone remodeling in maintenance of bone strength]
• [Spastin gene mutation in Chinese patients with hereditary spastic paraplegia]
• [Spectra analysis of [MnHg(SCN)4 (H2O)2 x 2C4H9NO (MMTWD) crystals]
• [Studies on the hydroxyl free radical-scavenging effect of combined selenium and germanium]
• [Study on molecular phylogeny of Schistosoma sinensium based on mitochondrial genes]