Isolation and characterization of two korean mistletoe lectins.

Isolation and characterization of two korean mistletoe lectins. Research Abstract Details 

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Isolation and characterization of two korean mistletoe lectins. Abstract Text:

Tae Bong Kang,Seong Kyu Song,Taek Joon Yoon,Yung Choon Yoo,Kwan Hee Lee,Erk Her,Jong Bae Kim,Tae Bong Kang,Seong Kyu Song,Taek Joon Yoon,Yung Choon Yoo,Kwan Hee Lee,Erk Her,Jong Bae Kim,Tae Bong Kang,Seong Kyu Song,Taek Joon Yoon,Yung Choon Yoo,Kwan Hee Lee,Erk Her,Jong Bae Kim,

Two isolectins (KML-IIU and the KML-IIL) were individually isolated from the previously reported Korean mistletoe lectin, KML-C, by using an immunoaffinity column. Molecular weights of the KML-IIU and the KML-IIL were 64 kDa and 60 kDa respectively. Both of the lectins were composed of heterogeneous A and B subunits linked with a disulfide bond, and showed the same carbohydrate-binding specificities for Gal and GalNAc. However, they are different not only in biophysical properties (glycosylation and amino acid compositions) but also bioactivities (cell killing and cytokine induction). The KML-IIL showed 17-145 times stronger in cytotoxicities to various human and mouse cancer cell lines than the KML-IIU. The KML-IIL also induced TNF-alpha secretion from mouse peritoneal macrophages 4.5 times better than the KML-IIU. The results demonstrated isolectins in Korean mistletoe were varied in bioactivities and the KML-IIL may be developed as an anti-cancer agent.

Isolation and characterization of two korean mistletoe lectins. Publishing Authors By Initials

TB Kang,SK Song,TJ Yoon,YC Yoo,KH Lee,E Her,JB Kim,TB Kang,SK Song,TJ Yoon,YC Yoo,KH Lee,E Her,JB Kim,TB Kang,SK Song,TJ Yoon,YC Yoo,KH Lee,E Her,JB Kim,

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Isolation and characterization of two korean mistletoe lectins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry and molecular biology

VOLUME: 40

Page Numbers: 959-65

Journal Abbreviation: J. Biochem. Mol. Biol.

ISSN: 1225-8687

DAY: 30

MONTH: Nov

YEAR: 2007

Isolation and characterization of two korean mistletoe lectins. Information

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LANGUAGE: eng

NlmUniqueID: 9702084

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Grant and Affiliation Information for Isolation and characterization of two korean mistletoe lectins.

AFFILIATION: School of Food and Biosciences, Handong Global University, Pohang 791-940, Korea jbkim@handong.edu.

Country: Korea (South)

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MEDLINETA: J Biochem Mol Biol

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