Isolation and characterization of multipotent human periodontal ligament stem cells.

Isolation and characterization of multipotent human periodontal ligament stem cells. Research Abstract Details 

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Isolation and characterization of multipotent human periodontal ligament stem cells. Abstract Text:

I C Gay,S Chen,M MacDougall,

BACKGROUND: Periodontal ligament (PDL) repair is thought to involve mesenchymal progenitor cells capable of forming fibroblasts, osteoblasts and cementoblasts. However, full characterization of PDL stem cell (SC) populations has not been achieved. OBJECTIVE: To isolate and characterize PDLSC and assess their capability to differentiate into bone, cartilage and adipose tissue. METHODS: Human PDL cells were stained for STRO-1, FACS sorted and expanded in culture. Human bone marrow SC (BMSC) served as a positive control. PDLSC and BMSC were cultured using standard conditions conducive for osteogenic, chondrogenic and adipogenic differentiation. Osteogenic induction was assayed using alizarine red S staining and expression of alkaline phosphatase (ALP) and bone sialoprotein (BSP). Adipogenic induction was assayed using Oil Red O staining and the expression of PPAR gamma 2 (early) and LPL (late) adipogenic markers. Chondrogenic induction was assayed by collagen type II expression and toluidine blue staining. RESULTS: Human PDL tissue contains about 27% STRO-1 positive cells with 3% strongly positive. In osteogenic cultures ALP was observed by day-7 in BMSC and day-14 in PDLSC. BSP expression was detectable by day-7; with more intense staining in PDLSC cultures. In adipogenic cultures both cell populations showed positive Oil Red O staining by day-25 with PPAR gamma 2 and LPL expression. By day-21, both BMSC and PDLSC chondrogenic induced cultures expressed collagen type II and glycosaminoglycans. CONCLUSIONS: The PDL contains SC that have the potential to differentiate into osteoblasts, chondrocytes and adipocytes, comparable with previously characterized BMSC. This adult PDLSC population can be utilized for potential therapeutic procedures related to PDL regeneration.

Isolation and characterization of multipotent human periodontal ligament stem cells. Publishing Authors By Initials

IC Gay,S Chen,M MacDougall,

For similar sialoglycoproteins research abstracts see: sialoglycoproteins research

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Isolation and characterization of multipotent human periodontal ligament stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Orthodontics & craniofacial research

VOLUME: 10

Page Numbers: 149-60

Journal Abbreviation:

ISSN: 1601-6335

DAY: 3

MONTH: Aug

YEAR: 2007

Isolation and characterization of multipotent human periodontal ligament stem cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101144387

Isolation and characterization of multipotent human periodontal ligament stem cells. Keywords Mesh Terms:

KEYWORDS: Sialoglycoproteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Isolation and characterization of multipotent human periodontal ligament stem cells. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Isolation and characterization of multipotent human periodontal ligament stem cells.

AFFILIATION: Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, AL 35294-0007, USA.

Country: England

AGENCY: United States NCI

GRANT: DE14318CO-STAR

ACRONYM: CO

MEDLINETA: Orthod Craniofac Res

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