Isolation and characterization of mammalian D-aspartyl endopeptidase.

Isolation and characterization of mammalian D-aspartyl endopeptidase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Isolation and characterization of mammalian D-aspartyl endopeptidase. Abstract Text:

T Kinouchi,H Nishio,Y Nishiuchi,M Tsunemi,K Takada,T Hamamoto,Y Kagawa,N Fujii,

The accumulation of D-isomers of aspartic acid (D-Asp) in proteins during aging has been implicated in the pathogenesis of Alzheimer's disease (AD), cataracts and arteriosclerosis. Here, we identified a specific lactacystin-sensitive endopeptidase that cleaves the D-Asp-containing protein and named it D-aspartyl endopeptidase (DAEP). DAEP has a multi-complex structure (MW: 600 kDa) and is localized in the inner mitochondrial membrane. However, DAEP activity was not detected in E. coli, S. cerevisiae, and C. elegans. A specific inhibitor for DAEP, i-DAEP: (benzoyl-L-Arg-L-His-[D-Asp]-CH(2)Cl; MW: 563.01), was newly synthesized and inhibited DAEP activity (IC(50), 3 microM), a factor of ten greater than lactacystin on DAEP. On the other hand, i-DAEP did not inhibit either the 20S or 26S proteasome. And we identified succinate dehydrogenase and glutamate dehydrogenase 1 as components of DAEP by affinity label using biotinylated i-DAEP. In the long life span of mammals, DAEP may serve as a scavenger against accumulation of racemized proteins in aging. Insights into DAEP will provide the foundation for developing treatments of diseases, such as AD, in which accumulation of D-Asp-containing proteins are implicated.

Isolation and characterization of mammalian D-aspartyl endopeptidase. Publishing Authors By Initials

T Kinouchi,H Nishio,Y Nishiuchi,M Tsunemi,K Takada,T Hamamoto,Y Kagawa,N Fujii,

For similar macromolecular substances: multiprotein complexes: multienzyme complexes: succinate cytochrome c oxidoreductase: electron transport complex ii: succinate dehydrogenase research abstracts see: macromolecular substances: multiprotein complexes: multienzyme complexes: succinate cytochrome c oxidoreductase: electron transport complex ii: succinate dehydrogenase research

PUBMED ID PMID:

MEDLINE DATE:

Isolation and characterization of mammalian D-aspartyl endopeptidase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Amino acids

VOLUME: 32

Page Numbers: 79-85

Journal Abbreviation: Amino Acids

ISSN: 0939-4451

DAY: 6

MONTH: 10

YEAR: 2006

Isolation and characterization of mammalian D-aspartyl endopeptidase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9200312

Isolation and characterization of mammalian D-aspartyl endopeptidase. Keywords Mesh Terms:

KEYWORDS: Succinate Dehydrogenase

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Isolation and characterization of mammalian D-aspartyl endopeptidase. Information

Substance Name: Proteasome Endopeptidase Complex

Registry Number: EC 3.4.25.1

Grant and Affiliation Information for Isolation and characterization of mammalian D-aspartyl endopeptidase.

AFFILIATION: Department of Radiation Life Science and Radiation Medical Science, Research Reactor Institute, Kyoto University, Osaka, Japan. kinouchi@HL.rri.kyoto-u.ac.jp

Country: Austria

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Amino Acids

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Isolation and characterization of mammalian D-aspartyl endopeptidase Related Publications

• CCND1 messenger RNA expression is correlated with EGFR mutation status in lung cancer.
• Cell type-specific roles of Jak3 in IL-2-induced proliferative signal transduction.
• Characterization of neutral fragments issued from the photodissociation of protonated tryptophane.
• Comprehensive characterization of the photodissociation pathways of protonated tryptophan.
• Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line.
• Cross-presentation of glycolipid from tumor cells loaded with alpha-galactosylceramide leads to potent and long-lived T cell mediated immunity via dendritic cells.
• Development of multilocus single strand conformation polymorphism (MLSSCP) analysis of virulence genes of Listeria monocytogenes and comparison with existing DNA typing methods.
• Dissimilatory iodate reduction by marine Pseudomonas sp. strain SCT.
• Effects of BCAA Supplementation on Makers of Muscle Damage and Inflammation during Low-Intensity Prolonged Exercise: 2070: Board #140 May 31 3:30 PM 5:00 PM.
• Effects of smoking on odour identification in Japanese subjects.
• Expression of ECRG4 is an independent prognostic factor for poor survival in patients with esophageal squamous cell carcinoma.
• High prevalence of erythromycin resistance and macrolide-resistance genes, mefA and ermB, in Streptococcus pneumoniae isolates from the upper respiratory tracts of children in the Sapporo district, Japan.
• Hydrogen desorption mechanism in a Li-N-H system by means of the isotopic exchange technique.
• In situ observation of phase transformation in an Fe-Zn system at high temperatures using an image plate.
• Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein.
• Isolation and characterization of mammalian D-aspartyl endopeptidase.
• Lacunar skull deformity and hydrocephalus in infants with myelomeningocele: is lacunar skull deformity a predictor of hydrocephalus development?
• Late intrathoracic relapse of pineal germinoma connected to intraspinal canal.
• Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler.
• Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress.
• PIK3CA gene amplification in Japanese non-small cell lung cancer.
• Phytoestrogens from Aspalathus linearis.
• Predictors of hepatic venous trunk invasion and prognostic factors in patients with hepatocellular carcinomas that had come into contact with the trunk of major hepatic veins.
• Primary malignant lymphoma of the cranial vault.
• Prognostic value of Ki-67 (MIB-1) and p53 in ependymomas.
• Ranitidine-induced drug eruption: another case of papuloerythroderma?
• Redundant promoter elements mediate IL-3-induced expression of a novel cytokine-inducible gene, cyclon.
• Regulation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase.
• Secretin-stimulating MRCP in patients with pancreatobiliary maljunction and occult pancreatobiliary reflux: direct demonstration of pancreatobiliary reflux.
• Temporal regulation of Stat5 activity in determination of cell differentiation program.