Isolation and characterization of kidney-derived stem cells.

Isolation and characterization of kidney-derived stem cells. Research Abstract Details 

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Isolation and characterization of kidney-derived stem cells. Abstract Text:

Sandeep Gupta,Catherine Verfaillie,David Chmielewski,Stefan Kren,Keith Eidman,Jeffrey Connaire,Yves Heremans,Troy Lund,Mark Blackstad,Yuehua Jiang,Aernout Luttun,Mark E Rosenberg,

Acute kidney injury is followed by regeneration of damaged renal tubular epithelial cells. The purpose of this study was to test the hypothesis that renal stem cells exist in the adult kidney and participate in the repair process. A unique population of cells that behave in a manner that is consistent with a renal stem cell were isolated from rat kidneys and were termed multipotent renal progenitor cells (MRPC). Features of these cells include spindle-shaped morphology; self-renewal for >200 population doublings without evidence for senescence; normal karyotype and DNA analysis; and expression of vimentin, CD90 (thy1.1), Pax-2, and Oct4 but not cytokeratin, MHC class I or II, or other markers of more differentiated cells. MRPC exhibit plasticity that is demonstrated by the ability of the cells to be induced to express endothelial, hepatocyte, and neural markers by reverse transcriptase-PCR and immunohistochemistry. The cells can differentiate into renal tubules when injected under the capsule of an uninjured kidney or intra-arterially after renal ischemia-reperfusion injury. Oct4 expression was seen in some tubular cells in the adult kidney, suggesting these cells may be candidate renal stem cells. It is proposed that MRPC participate in the regenerative response of the kidney to acute injury.

Isolation and characterization of kidney-derived stem cells. Publishing Authors By Initials

S Gupta,C Verfaillie,D Chmielewski,S Kren,K Eidman,J Connaire,Y Heremans,T Lund,M Blackstad,Y Jiang,A Luttun,ME Rosenberg,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred f344 research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred f344 research

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Isolation and characterization of kidney-derived stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Society of Nephrology : JA

VOLUME: 17

Page Numbers: 3028-40

Journal Abbreviation: J. Am. Soc. Nephrol.

ISSN: 1046-6673

DAY: 20

MONTH: 09

YEAR: 2006

Isolation and characterization of kidney-derived stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 9013836

Isolation and characterization of kidney-derived stem cells. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred F344

MESH TERMS: cytology

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Grant and Affiliation Information for Isolation and characterization of kidney-derived stem cells.

AFFILIATION: Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA. gupta024@umn.edu

Country: United States

AGENCY: United States NIDDK

GRANT: DK68470

ACRONYM: DK

MEDLINETA: J Am Soc Nephrol

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