Isolation and Characterization of Extracellular alpha-Galactosidases from Penicillium canescens.

Isolation and Characterization of Extracellular alpha-Galactosidases from Penicillium canescens. Research Abstract Details 

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Isolation and Characterization of Extracellular alpha-Galactosidases from Penicillium canescens. Abstract Text:

O A Sinitsyna,E A Fedorova,I M Vakar,E G Kondratieva,A M Rozhkova,L M Sokolova,T M Bubnova,O N Okunev,A M Chulkin,Y P Vinetsky,A P Sinitsyn,

Two alpha-galactosidases were purified to homogeneity from the enzymatic complex of the mycelial fungus Penicillium canescens using chromatography on different sorbents. Substrate specificity, pH- and temperature optima of activity, stability under different pH and temperature conditions, and the influence of effectors on the catalytic properties of both enzymes were investigated. Genes aglA and aglC encoding alpha-galactosidases from P. canescens were isolated, and amino acid sequences of the proteins were predicted. In vitro feed testing (with soybean meal and soybean byproducts enriched with galactooligosaccharides as substrates) demonstrated that both alpha-galactosidases from P. canescens could be successfully used as feed additives. alpha-Galactosidase A belonging to the 27th glycosyl hydrolase family hydrolyzed galactopolysaccharides (galactomannans) and alpha-galactosidase C belonging to the 36th glycosyl hydrolase family hydrolyzed galactooligosaccharides (stachyose, raffinose, etc.) of soybean with good efficiency, thus improving the digestibility of fodder.

Isolation and Characterization of Extracellular alpha-Galactosidases from Penicillium canescens. Publishing Authors By Initials

OA Sinitsyna,EA Fedorova,IM Vakar,EG Kondratieva,AM Rozhkova,LM Sokolova,TM Bubnova,ON Okunev,AM Chulkin,YP Vinetsky,AP Sinitsyn,

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Isolation and Characterization of Extracellular alpha-Galactosidases from Penicillium canescens. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemistry. Biokhimii?a

VOLUME: 73

Page Numbers: 97-106

Journal Abbreviation: Biochemistry Mosc.

ISSN: 0006-2979

DAY: 25

MONTH: Jan

YEAR: 2008

Isolation and Characterization of Extracellular alpha-Galactosidases from Penicillium canescens. Information

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LANGUAGE: eng

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AFFILIATION: Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia. oasinitsyna@gmail.com.

Country: United States

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MEDLINETA: Biochemistry (Mosc)

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