Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica.

Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Research Abstract Details 

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Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Abstract Text:

H Tsuchihashi,T Yadomae,T Miyazaki,

An endo-beta-D-glucuronidase was isolated and characterized from Kobayasia nipponica. The enzyme was purified by ammonium sulfate fractionation, CM-Sephadex chromatography, gel filtration with Sephacryl S-200, and heparin-Sepharose chromatography. The enzyme shows the following properties: optimum pH 5.0, thermal stability below 37 degrees C, pH stability 5-6, optimum temperature 45-55 degrees C, and Km 0.12% for L-idurono-D-glucuronan (protuberic acid (PA), L-IdUA:D-GlcUA = 1:2) from Kobayasia nipponica, 0.19% for PF (L-IdUA:D-GlcUA = 1:3) from Pseudocolus fusiformis, and 0.23% for (1-4)-beta-D-glucuronan(mucoric acid) from Mucor mucedo as determined from Hofstee plots. The molecular weight values estimated by gel filtration through Sephacryl S-200 and Sephadex G-50 and by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate were 10,500 and 10,200, respectively. The endo-beta-D-glucuronidase was inactive towards several glycosaminoglycans.

Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Publishing Authors By Initials

H Tsuchihashi,T Yadomae,T Miyazaki,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 1799-805

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1984

Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Isolation and characterization of an endo-beta-D-glucuronidase from the fungus Kobayasia nipponica. Information

Substance Name: Glucuronidase

Registry Number: EC 3.2.1.31

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Country: JAPAN

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MEDLINETA: J Biochem

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