Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper.

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper. Research Abstract Details 

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Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper. Abstract Text:

A V ,A Rucavado,L Sanz,J J Calvete,J M ,

A serine proteinase with thrombin-like activity was isolated from the venom of the Central American pit viper Bothrops asper. Isolation was performed by a combination of affinity chromatography on aminobenzamidine-Sepharose and ion-exchange chromatography on DEAE-Sepharose. The enzyme accounts for approximately 0.13% of the venom dry weight and has a molecular mass of 32 kDa as determined by SDS-PAGE, and of 27 kDa as determined by MALDI-TOF mass spectrometry. Its partial amino acid sequence shows high identity with snake venom serine proteinases and a complete identity with a cDNA clone previously sequenced from this species. The N-terminal sequence of the enzyme is VIGGDECNINEHRSLVVLFXSSGFL CAGTLVQDEWVLTAANCDSKNFQ. The enzyme induces clotting of plasma (minimum coagulant dose = 4.1 microg) and fibrinogen (minimum coagulant dose = 4.2 microg) in vitro, and promotes defibrin(ogen)ation in vivo (minimum defibrin(ogen)ating dose = 1.0 microg). In addition, when injected intravenously in mice at doses of 5 and 10 microg, it induces a series of behavioral changes, i.e., loss of the righting reflex, opisthotonus, and intermittent rotations over the long axis of the body, which closely resemble the ;gyroxin-like' effect induced by other thrombin-like enzymes from snake venoms.

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper. Publishing Authors By Initials

AV ,A Rucavado,L Sanz,JJ Calvete,JM ,

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Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Brazilian journal of medical and biological resear

VOLUME: 41

Page Numbers: 12-7

Journal Abbreviation: Braz. J. Med. Biol. Res.

ISSN: 0100-879X

DAY: 17

MONTH: 12

YEAR: 2007

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper. Information

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LANGUAGE: eng

NlmUniqueID: 8112917

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Grant and Affiliation Information for Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper.

AFFILIATION: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.

Country: Brazil

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MEDLINETA: Braz J Med Biol Res

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