Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis.

Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis. Abstract Text:

Jae Cheol Jeong,Dongjin Shin,Jiyoung Lee,Chang Ho Kang,Dongwon Baek,Moo Je Cho,Min Chul Kim,Dae-Jin Yun,Jae Cheol Jeong,Dongjin Shin,Jiyoung Lee,Chang Ho Kang,Dongwon Baek,Moo Je Cho,Min Chul Kim,Dae-Jin Yun,

Protein phosphorylation is one of the major mechanisms by which eukaryotic cells transduce extracellular signals into intracellular responses. Calcium/calmodulin (Ca(2+)/CaM)-dependent protein phosphorylation has been implicated in various cellular processes, yet little is known about Ca(2+)/CaM-dependent protein kinases (CaMKs) in plants. From an Arabidopsis expression library screen using a horseradish peroxidase-conjugated soybean calmodulin isoform (SCaM-1) as a probe, we isolated a full-length cDNA clone that encodes AtCK (Arabidopsis thaliana calcium/calmodulin-dependent protein kinase). The predicted structure of AtCK contains a serine/threonine protein kinase catalytic domain followed by a putative calmodulin-binding domain and a putative Ca(2+)-binding domain. Recombinant AtCK was expressed in E. coli and bound to calmodulin in a Ca(2+)-dependent manner. The ability of CaM to bind to AtCK was confirmed by gel mobility shift and competition assays. AtCK exhibited its highest levels of autophosphorylation in the presence of 3 mM Mn(2+). The phosphorylation of myelin basic protein (MBP) by AtCK was enhanced when AtCK was under the control of calcium-bound CaM, as previously observed for other Ca(2+)/CaM-dependent protein kinases. In contrast to maize and tobacco CCaMKs (calcium and Ca(2+)/CaM-dependent protein kinase), increasing the concentration of calmodulin to more than 3 microgram suppressed the phosphorylation activity of AtCK. Taken together our results indicate that AtCK is a novel Arabidopsis Ca(2+)/CaM-dependent protein kinase which is presumably involved in CaM-mediated signaling.

Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis. Publishing Authors By Initials

JC Jeong,D Shin,J Lee,CH Kang,D Baek,MJ Cho,MC Kim,DJ Yun,JC Jeong,D Shin,J Lee,CH Kang,D Baek,MJ Cho,MC Kim,DJ Yun,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecules and cells

VOLUME: 24

Page Numbers: 276-82

Journal Abbreviation: Mol. Cells

ISSN: 1016-8478

DAY: 31

MONTH: Oct

YEAR: 2007

Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9610936

Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis.

AFFILIATION: Division of Applied Life Sciences (BK21 program), Plant Molecular Biology & Biotechnology Research Center, Graduate School of Gyeongsang National University, Jinju 660-701, Korea.

Country: Korea (South)

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Cells

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis Related Publications

• Antiplatelet activity of hesperetin, a bioflavonoid, is mainly mediated by inhibition of PLC-gamma2 phosphorylation and cyclooxygenase-1 activity.
• Antiplatelet effect of NQ12: a possible mechanism through the arachidonic acid cascade.
• Consequences of birth policies and practices in post-reform china.
• Cudraflavanone A, a flavonoid isolated from the root bark of Cudrania tricuspidata, inhibits vascular smooth muscle cell growth via an Akt-dependent pathway.
• Epothilone D, a microtubule-stabilizing compound, inhibits neointimal hyperplasia after rat carotid artery injury by cell cycle arrest via regulation of G1-checkpoint proteins.
• Isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, AtCK, from arabidopsis.
• Kawasaki disease in Korea, 2003-2005.
• Lateral segregation of anionic phospholipids in model membranes induced by cytochrome P450 2B1: bi-directional coupling between CYP2B1 and anionic phospholipid.
• Lysophosphatidic acid prevents apoptosis of Caco-2 colon cancer cells via activation of mitogen-activated protein kinase and phosphorylation of Bad.
• Management of ocular surface inflammation in Sjögren syndrome.
• New and efficient method using Saccharomyces cerevisiae mutants for identification of siderophores produced by microorganisms.
• New antioxidant polyphenols from the medicinal mushroom Inonotus obliquus.
• New triterpene glucosides, oligoporins A-C, from Oligoporus tephroleucus protect DNA from Fenton reaction.
• Optical coherence tomography using rapidly swept lasers.
• Pharmacokinetics of florfenicol and its metabolite, florfenicol amine, in dogs.
• Phase separation in phosphatidylcholine/anionic phospholipid membranes in the liquid-crystalline state revealed with fluorescent probes.
• Physical and functional interaction of FgFtr1-FgFet1 and FgFtr2-FgFet2 is required for iron uptake in Fusarium graminearum.
• Prevalence of Eperythrozoon spp. infection and congenital eperythrozoonosis in humans in Inner Mongolia, China.
• Quality of life and sexual problems in disease-free survivors of cervical cancer compared with the general population.
• Roquinimex-mediated protection effect on the development of chronic graft-versus-host disease in mice is associated with induction of Th1 cytokine production and inhibition of proinflammatory cytokine production.
• Simple and sensitive method for determination of nitrated polycyclic aromatic hydrocarbons in diesel exhaust particles by gas chromatography-negative ion chemical ionisation tandem mass spectrometry.
• Sphingosine kinase 1 expression is regulated by signaling through PI3K, AKT2, and mTOR in human coronary artery smooth muscle cells.
• Tetraaqua-1kappaO,2kappa3O-(mu-2,4-dinitrophenolato-1kappa2O1,O2:2kappaO1)(2,4-dinitrophenolato-1kappa2O1,O2)dilithium(I): a dinuclear lithium(I) complex.
• Toxicity of imidazolium salt with anion bromide to a phytoplankton Selenastrum capricornutum: Effect of alkyl-chain length.
• [A case of infant infected by Blastocystis hominis]
• [Clinical study of treatment nonalcoholic fatty liver with qianggan capsule]
• [Effects of rhamnolipid on the biodegradation of n-hexadecane by microorganism and the cell surface hydrophobicity]
• [Pilot study for the inclusion of the portfolio of the family medicine specialist book in training.]
• [Study on reversed-phase ion-pair chromatographic determination of thiosulfate and iodide anions]
• [The stability of carbon monoxide in stored blood samples]