Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity.

Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity. Research Abstract Details 

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Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity. Abstract Text:

Jian Wang,Guohua Chen,Julie Lee,Kostas Pantopoulos,

BACKGROUND: Iron regulatory protein 2 (IRP2), a post-transcriptional regulator of cellular iron metabolism, undergoes iron-dependent degradation via the ubiquitin-proteasome pathway. A stretch of 73 amino acids within the N-terminal domain 1 of the protein was reported to function as an iron sensor. However, mutants lacking this fragment remain sensitive to degradation in iron-replete cells. RESULTS: To identify elements within IRP2 involved in the control of its stability, we undertook a systematic mutagenesis approach. Truncated versions of IRP2 were expressed in H1299 cells and analyzed for their response to iron. Deletion mutants lacking the entire C-terminal domain 4 (amino acids 719-963) of IRP2 remained stable following iron treatments. Moreover, the replacement of domain 4 of IRP1 with the corresponding region of IRP2 sensitized the chimerical IRP11-3/IRP24 protein to iron-dependent degradation, while the reverse manipulation gave rise to a stable chimerical IRP21-3/IRP14 protein. The deletion of just 26 or 34 C-terminal amino acids stabilized IRP2 against iron. However, the fusion of C-terminal IRP2 fragments to luciferase failed to sensitize the indicator protein for degradation in iron-loaded cells. CONCLUSION: Our data suggest that the C-terminus of IRP2 contains elements that are necessary but not sufficient for iron-dependent degradation. The functionality of these elements depends upon the overall IRP structure.

Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity. Publishing Authors By Initials

J Wang,G Chen,J Lee,K Pantopoulos,

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Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC molecular biology

VOLUME: 9

Page Numbers: 15

Journal Abbreviation: BMC Mol. Biol.

ISSN: 1471-2199

DAY: 28

MONTH: 01

YEAR: 2008

Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity. Information

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LANGUAGE: eng

NlmUniqueID: 100966983

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AFFILIATION: Lady Davis Institute for Medical Research, Sir Mortimer B, Davis Jewish General Hospital, Montreal, Quebec, Canada. jian.wang@utsouthwestern.edu

Country: England

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MEDLINETA: BMC Mol Biol

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