Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity.

Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity. Research Abstract Details 

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Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity. Abstract Text:

Lixin Li,Balz Frei,

OBJECTIVE: Excess iron may increase oxidative stress and play a role in vascular inflammation and atherosclerosis. Here we determined whether the iron chelator, desferrioxamine (DFO), ameliorates oxidative stress and cellular adhesion molecule expression in a murine model of local inflammation. METHODS AND RESULTS: Dorsal air pouches were created in C57BL/6J mice by subcutaneous injection of air. DFO (100 mg/kg body weight) was injected into the air pouch once a day for two days followed immediately on the second day by lipopolysaccharide (LPS; 2.5 mg/kg body weight). The animals were euthanized 24 hours later for analysis of oxidative stress markers and adhesion molecules in air pouch tissue. LPS treatment enhanced protein levels of p22(phox), a catalytic subunit of NADPH oxidase, and increased NADPH oxidase activity and levels of superoxide radicals and hydrogen peroxide. Furthermore, LPS activated NF-kappaB and increased expression of adhesion molecules. All of these inflammatory responses were strongly suppressed by DFO, but not iron-loaded DFO. CONCLUSIONS: Our data show that DFO inhibits LPS-induced, NADPH oxidase-mediated oxidative stress and, hence, NF-kappaB activation and adhesion molecule expression in a murine model of local inflammation. Iron chelation may be helpful in treating atherosclerotic vascular diseases by ameliorating oxidative stress and inflammation.

Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity. Publishing Authors By Initials

L Li,B Frei,

For similar musculoskeletal system: skeleton: joints: joint capsule: synovial membrane research abstracts see: musculoskeletal system: skeleton: joints: joint capsule: synovial membrane research

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Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Arteriosclerosis, thrombosis, and vascular biology

VOLUME: 26

Page Numbers: 2638-43

Journal Abbreviation: Arterioscler. Thromb. Vasc. Bi

ISSN: 1524-4636

DAY: 14

MONTH: 09

YEAR: 2006

Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9505803

Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity. Keywords Mesh Terms:

KEYWORDS: Synovial Membrane

MESH TERMS: pathology

Chemical & Substance for Abstract: Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity. Information

Substance Name: p22(phox) protein, mouse

Registry Number: EC 1.6.3.1

Grant and Affiliation Information for Iron chelation inhibits NF-kappaB-mediated adhesion molecule expression by inhibiting p22(phox) protein expression and NADPH oxidase activity.

AFFILIATION: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331-6512, USA.

Country: United States

AGENCY: United States NCCAM

GRANT: P01 AT002034

ACRONYM: AT

MEDLINETA: Arterioscler Thromb Vasc Biol

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