Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface.

Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. Research Abstract Details 

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Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. Abstract Text:

Jason S Page,Ryan T Kelly,Keqi Tang,Richard D Smith,

The ionization and transmission efficiencies of an electrospray ionization (ESI) interface were investigated to advance the understanding of how these factors affect mass spectrometry (MS) sensitivity. In addition, the effects of the ES emitter distance to the inlet, solution flow rate, and inlet temperature were characterized. Quantitative measurements of ES current loss throughout the ESI interface were accomplished by electrically isolating the front surface of the interface from the inner wall of the heated inlet capillary, enabling losses on the two surfaces to be distinguished. In addition, the ES current lost to the front surface of the ESI interface was spatially profiled with a linear array of 340-microm-diameter electrodes placed adjacent to the inlet capillary entrance. Current transmitted as gas-phase ions was differentiated from charged droplets and solvent clusters by measuring sensitivity with a single quadrupole mass spectrometer. The study revealed a large sampling efficiency into the inlet capillary (>90% at an emitter distance of 1 mm), a global rather than a local gas dynamic effect on the shape of the ES plume resulting from the gas flow conductance limit of the inlet capillary, a large (>80%) loss of analyte ions after transmission through the inlet arising from incomplete desolvation at a solution flow rate of 1.0 microL/min, and a decrease in analyte ions peak intensity at lower temperatures, despite a large increase in ES current transmission efficiency.

Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. Publishing Authors By Initials

JS Page,RT Kelly,K Tang,RD Smith,

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Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of the American Society for Mass Spectrome

VOLUME: 18

Page Numbers: 1582-90

Journal Abbreviation: J. Am. Soc. Mass Spectrom.

ISSN: 1044-0305

DAY: 2

MONTH: 06

YEAR: 2007

Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. Information

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LANGUAGE: eng

NlmUniqueID: 9010412

Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. Keywords Mesh Terms:

KEYWORDS: Transducers

MESH TERMS: methods

Chemical & Substance for Abstract: Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface. Information

Substance Name: Ions

Registry Number: 0

Grant and Affiliation Information for Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface.

AFFILIATION: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.

Country: United States

AGENCY: United States NIAID

GRANT: Y1-AI-4894-01

ACRONYM: AI

MEDLINETA: J Am Soc Mass Spectrom

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