Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate.

Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate. Research Abstract Details 

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Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate. Abstract Text:

T Takabe,S Niwa,H Ishikawa,M Miyakawa,

Kinetic studies of the reduction of plastocyanin from spinach chloroplasts by ascorbate were carried out using a stopped flow apparatus. The reduction of spinach plastocyanin by ascorbate followed first-order kinetics in the reductant with a rate constant k = 415 M-1.S-1 [20 degrees C, 0.1 M NaCl, pH 8.0 (10 mM Tris-HCl)]. The second-order rate constants for the reduction of plastocyanin by ascorbate increased dramatically with increasing pH and ionic strength. The cationic micelles of cetyltrimethylammonium bromide also accelerated this electron-transfer reaction. The importance of electrostatic interactions between plastocyanin and ascorbate is discussed in relation to the photosynthetic electron-transport reactions.

Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate. Publishing Authors By Initials

T Takabe,S Niwa,H Ishikawa,M Miyakawa,

For similar proteins: metalloproteins: plastocyanin research abstracts see: proteins: metalloproteins: plastocyanin research

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Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 111-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1980

Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate. Keywords Mesh Terms:

KEYWORDS: Plastocyanin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate. Information

Substance Name: Plastocyanin

Registry Number: 9014-09-9

Grant and Affiliation Information for Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate.

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Country: JAPAN

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MEDLINETA: J Biochem

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