[Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase]

[Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase] Abstract Text:

Zhaohua Chen,Shutao Liu,Weiming Xue,Weiping Lai,Pingfan Rao,

A rapid, simple and sensitive method was developed to characterize S. aureus in exponential phase by a high performance ion-exchange chromatograph equipped with a laser light-scattering detector. An ion-exchange column (TSKgel SuperQ-TOYOPEARL 650C column, 7.6 mm i.d. x 100 mm, Tosoh, Japan) was used for the separation. The mobile phase was a 20 mmol/L piperazine-hydrochloric acid (pH 6.0) buffer. It was found that S. aureus in exponential phase exhibited two elution peaks. Transmission electron microscope (TEM) showed that the bacterial cells in the first peak appeared to be circular, while the cells in the second one appeared to be elliptical and had one obvious diaphragm. It is found that the proliferation capability of the second fraction was better than the first fraction by 65.3%. These results suggested that the first fraction was the S. aureus that stopped dividing, while the second one was the S. aureus that were undergoing cell division. Therefore, the chromatographic method developed here may be a promising tool for the rapid and efficient separation and characterization of bacterial cells.

[Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase] Publishing Authors By Initials

Z Chen,S Liu,W Xue,W Lai,P Rao,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Se pu = Chinese journal of chromatography / Zhongg

VOLUME: 22

Page Numbers: 234-6

Journal Abbreviation:

ISSN: 1000-8713

DAY: 16

MONTH: May

YEAR: 2004

[Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 9424804

[Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase]

AFFILIATION: Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China. chzhaohua@163.com

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Se Pu

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase Related Publications

• A common neural progenitor for the CNS and PNS.
• Acute hemorrhagic pancreatic necrosis in mice. Influence of the age and sex of the animals and of dietary ethionine, choline, methionine, and adenine sulfate.
• Age-related functional recruitment for famous name recognition: an event-related fMRI study.
• An FMRI analysis of the human hippocampus: inference, context, and task awareness.
• An Intelligent 802.11 Triage Tag for medical response to disasters.
• Cellular and molecular guidance of GABAergic neuronal migration from an extracortical origin to the neocortex.
• Changes in Behavior and Brain Acetylcholinesterase Activity in Mosquito Fish, Gambusia affinis in Response to the Sub-Lethal Exposure to Chlorpyrifos.
• Development and Validation of Liquid Chromatographic and UV Derivative Spectrophotometric Methods for the Determination of Famciclovir in Pharmaceutical Dosage Forms.
• Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen.
• Embryonic expression and extracellular secretion of Xenopus slit.
• Evaluation of Cytotoxic Effects of Dichloromethane Extract of Guduchi (Tinospora cordifolia Miers ex Hook F & THOMS) on Cultured HeLa Cells.
• Inhibition by the bioflavonoid ternatin of aflatoxin B1-induced lipid peroxidation in rat liver.
• Ion imprinted polymer particles for separation of yttrium from selected lanthanides.
• Medial temporal lobe activity for recognition of recent and remote famous names: an event-related fMRI study.
• Outbreak of killer brain disease in children.
• Perspectives in medical education--1. Reflections on the state of medical education in Japan.
• Role of the chemokine SDF-1 as the meningeal attractant for embryonic cerebellar neurons.
• Slit proteins: molecular guidance cues for cells ranging from neurons to leukocytes.
• Spinal cord neuronal precursors generate multiple neuronal phenotypes in culture.
• THE ORNITHOPHILIC BLACK FLY SIMULIUM MERIDIONALE RILEY (DIPTERA: SIMULIIDAE) FEEDING IN MAN DURING AUTUMN.
• The expression of TNF-alpha mRNA and its significance in murine viral myocarditis.
• The neuronal repellent Slit inhibits leukocyte chemotaxis induced by chemotactic factors.
• The secreted product of Xenopus gene lunatic Fringe, a vertebrate signaling molecule.
• [Analysis of diffuse large B-cell lymphoma heterogeneity based on coupled two-way clustering]
• [Effect of acrylamide on creatine kinase and adenosine triphosphate in brain of mice and its significance]
• [Inhibitive effects of gastric cancer cell-dendritic cell fusion vaccine on tumor cell proliferation cycle]
• [Ion-exchange chromatographic behavior and characterization of Staphylococcus aureus in exponential phase]
• [Major constituent proteins in donkey hide and their interaction]
• [Reaction of atrazine with protein and its application to detection of protein]
• fMRI of healthy older adults during Stroop interference.