Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.

Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Research Abstract Details 

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Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Abstract Text:

Philippe Desharnais, ,Claudine Hamelin,Patrick Devine,Jacques Bernier,

CD45 is a type I transmembrane molecule with phosphatase activity which comprises up to 10% of the cell surface area in nucleated haematopoietic cells. We have previously demonstrated the absence of nuclear apoptosis in CD45-negative T cells after chemical-induced apoptosis. The aim of this study was to characterize the role of CD45 in nuclear apoptosis. In contrast to wild type CD45-positive T cells, the CD45-deficient T cell lines are resistant to the induction of DNA fragmentation and chromatin condensation following tributyltin (TBT) or H(2)O(2 )exposure, but not to cycloheximide-induced apoptosis. CD45 transfection in deficient cell lines led to the restoration of chromatin condensation and DNA fragmentation following TBT exposure. In both CD45-positive and negative T cell lines, TBT exposure mediates intracellular calcium mobilization, caspase-3 activation and DFF45 cleavage. Moreover, DNA fragmentation was also induced by TBT in cells deficient in expression of p56lck, ZAP-70 and SHP-1. Subcellular partitioning showed a decrease in nuclear localisation of caspase-3 and DFF40. Together, these results demonstrate for the first time, that CD45 expression plays a key role in internucleosomal DNA fragmentation and chromatin condensation processes during apoptosis. CD45 activity or its substrates' activity, appears to be located downstream of caspase-3 activation and plays a role in retention of DFF40 in the nucleus.

Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Publishing Authors By Initials

P Desharnais,G ,C Hamelin,P Devine,J Bernier,

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Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Apoptosis : an international journal on programmed

VOLUME: 13

Page Numbers: 197-212

Journal Abbreviation: Apoptosis

ISSN: 1360-8185

DAY: 30

MONTH: Feb

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 9712129

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Grant and Affiliation Information for Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.

AFFILIATION: INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, QC, Canada, H7V 1B7.

Country: United States

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MEDLINETA: Apoptosis

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