Involvement of calpain in osteoclastic bone resorption.

Involvement of calpain in osteoclastic bone resorption. Research Abstract Details

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Involvement of calpain in osteoclastic bone resorption. Abstract Text:

Masami Hayashi,Yasuko Koshihara,Hideaki Ishibashi,Seizo Yamamoto,Satoshi Tsubuki,Takaomi C Saido,Seiichi Kawashima,Mitsushi Inomata,

There is increasing evidence that calpain contributes to the reorganization of the cytoskeleton in the integrin-mediated signaling pathway. Osteoclastic bone resorption requires cell-matrix contact, an event mediated by integrin alphavbeta3, and subsequent cytoskeletal reorganization to form characteristic membrane domains such as the sealing zone and ruffled border. In this study, therefore, we investigated whether calpain is involved in osteoclastic bone resorption. Membrane-permeable calpain inhibitors suppress the resorption activity of human osteoclasts, but an impermeable inhibitor does not. Upon the attachment of osteoclasts to bone, micro-calpain is translocated from the cytosolic to the cytoskeletal fraction and is autolytically activated. Both the activation of micro-calpain and the formation of actin-rings, the cytoskeletal structures essential for bone resorption, are inhibited by membrane-permeable calpain inhibitors. The activated micro-calpain in osteoclasts selectively cleaves talin, which links the matrix-recognizing integrin to the actin cytoskeleton. These findings suggest that calpain is a regulator of the bone resorption activity of osteoclasts through reorganization of the cytoskeleton related to actin-ring formation.

Involvement of calpain in osteoclastic bone resorption. Publishing Authors By Initials

M Hayashi,Y Koshihara,H Ishibashi,S Yamamoto,S Tsubuki,TC Saido,S Kawashima,M Inomata,

For similar proteins: cytoskeletal proteins: talin research abstracts see: proteins: cytoskeletal proteins: talin research

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Involvement of calpain in osteoclastic bone resorption. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 137

Page Numbers: 331-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 2005

Involvement of calpain in osteoclastic bone resorption. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Involvement of calpain in osteoclastic bone resorption. Keywords Mesh Terms:

KEYWORDS: Talin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Involvement of calpain in osteoclastic bone resorption. Information

Substance Name: Calpain

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Involvement of calpain in osteoclastic bone resorption.

AFFILIATION: Biomembrane Research Group, and Bone Research Group, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015.

Country: Japan