Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis.

Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis. Research Abstract Details 

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Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis. Abstract Text:

T Yokota,K I Arai,Y Kaziro,

The effect of 30S ribosomal protein S1 on poly(U)-directed polyphenylalanine synthesis was studied using a highly purified cell-free system which was devoid of endogenous S1. The system consisted of homogeneous preparations of EF-Tu, EF-Ts, and EF-G, and 70S ribosomes from which protein S1 had been removed by poly(U)-cellulose column chromatography. It was found that protein S1 was indispensable for translation of poly(U) by an S1-depleted system at low concentrations of poly(U). On the other hand, at higher concentrations of poly(U), a considerable amount of polyphenylalanine was synthesized in the absence of added S1. The stimulatory effect of S1 was observed at all Mg2+ concentrations examined but was most pronounced at 10 mM Mg2+. Some physicochemical properties of the protein were also studied. It was demonstrated that the protein has an elongated shape with an axial ratio of approximately 8.5.

Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis. Publishing Authors By Initials

T Yokota,KI Arai,Y Kaziro,

For similar cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research abstracts see: cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research

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Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 82

Page Numbers: 1485-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1977

Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis. Keywords Mesh Terms:

KEYWORDS: Ribosomes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis. Information

Substance Name: Magnesium

Registry Number: 7439-95-4

Grant and Affiliation Information for Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis.

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Country: JAPAN

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MEDLINETA: J Biochem

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