Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry.

Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry. Research Abstract Details 

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Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry. Abstract Text:

Igor A Kaltashov,Mingxuan Zhang,Stephen J Eyles,Rinat R Abzalimov,

Electrospray ionization mass spectrometry (ESI MS) has emerged recently as a powerful tool for analyzing many structural and behavioral aspects of metalloproteins in great detail. In this review we discuss recent developments in the field, placing particular emphasis on the unique features of ESI MS that lend themselves to metalloprotein characterization at a variety of levels. Direct mass measurement enables the determination of protein-metal ion binding stoichiometry in solution and metalloprotein higher order structure in the case of multi-subunit proteins. MS techniques have been developed for determining the locations of metal-binding centers, metal oxidation states and reaction intermediates of metal-containing enzymes. Other ESI MS techniques are also discussed, such as protein ion charge state distributions and hydrogen/deuterium exchange studies, which can be used to measure metal binding affinities and to shed light on vital dynamic aspects of the functional properties of metalloproteins endowed by metal binding.

Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry. Publishing Authors By Initials

IA Kaltashov,M Zhang,SJ Eyles,RR Abzalimov,

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Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry. Journal Published:

PUBLICATION TYPE: Review

Journal: Analytical and bioanalytical chemistry

VOLUME: 386

Page Numbers: 472-81

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ISSN: 1618-2642

DAY: 25

MONTH: 08

YEAR: 2006

Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry. Information

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LANGUAGE: eng

NlmUniqueID: 101134327

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AFFILIATION: Department of Chemistry, University of Massachusetts-Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA. kaltashov@chem.umass.edu

Country: Germany

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MEDLINETA: Anal Bioanal Chem

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