Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry.

Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry. Research Abstract Details 

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Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry. Abstract Text:

Xuguang Yan,Max L Deinzer,Michael I Schimerlik,David Broderick,Mark E Leid,Marcia I Dawson,

Several different agonists of the retinoic X receptor alpha (hRXRalpha) were examined for their effects on the amide H/D exchange kinetics of the homodimeric protein using mass spectrometry. Some agonists, LG 100268, SR11246, and DHA, bind such that slower deuterium exchange-in occurs compared with 9-cis-retinoic acid (9-cis-RA), whereas others, fenretinide and methoprenic acid, result in poorer protection during binding and hence faster exchange-in. Protection against H/D exchange by different agonists and the inhibition of H/D exchange kinetics relative to 9-cis-RA varies markedly in different regions of the protein. Agonists LG 100268, SR11246, and DHA generally inhibit faster exchange processes in the ligand binding regions of hRXRalpha than does the native ligand 9-cis-RA. In at least half of these regions, the level of protection by 9-cis-RA lags behind the agonists even after 60 min. Methoprenic acid did not significantly protect hRXRalpha against amide hydrogen exchange. An efficient method is described for comparing the effects of different agonists on the protein structure of the agonist-RXRalpha complex.

Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry. Publishing Authors By Initials

X Yan,ML Deinzer,MI Schimerlik,D Broderick,ME Leid,MI Dawson,

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Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of the American Society for Mass Spectrome

VOLUME: 17

Page Numbers: 1510-7

Journal Abbreviation: J. Am. Soc. Mass Spectrom.

ISSN: 1044-0305

DAY: 26

MONTH: 07

YEAR: 2006

Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9010412

Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Electrospray Ionizat

MESH TERMS: chemistry

Chemical & Substance for Abstract: Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry. Information

Substance Name: Hydrogen

Registry Number: 1333-74-0

Grant and Affiliation Information for Investigation of ligand interactions with human RXRalpha by hydrogen/deuterium exchange and mass spectrometry.

AFFILIATION: Department of Chemistry, Oregon State University, Corvallis, Oregon 94331, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: ES00040

ACRONYM: ES

MEDLINETA: J Am Soc Mass Spectrom

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