Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo.

Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. Research Abstract Details 

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Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. Abstract Text:

Shinsuke Fujii,Hidefumi Maeda,Naohisa Wada,Atsushi Tomokiyo,Masahiro Saito,Akifumi Akamine,

The lifespan of the tooth is influenced by the periodontal ligament (PDL), a specialized connective tissue that connects the cementum with the tooth socket bone. Generation of a cell line from PDL progenitor/stem cells would allow development of tissue engineering-based regenerative PDL therapy. However, little is known about the characteristics of PDL progenitor/stem cells because PDL tissue consists of a heterogeneous cell population and there are no pure PDL cell lines. Recently, we succeeded in immortalizing primary human PDL fibroblasts (HPLFs) by transfecting them with SV40 T-antigen and hTERT (Cell Tissue Res 2006; 324: 117-125). In this study, we isolated three clonal cell lines from these immortalized cells (lines 1-4, 1-11, and 1-24) that express RUNX-2, Col I, ALP, OPN, OCN, RANKL, OPG, scleraxis, periostin, Col XII, and alpha-SMA mRNA. Immunocytochemical analysis demonstrated that CD146 was expressed in cell lines 1-4 and 1-11 and that STRO-1 was expressed in lines 1-11 and 1-24. Lines 1-4 and 1-11 differentiated into osteoblastic cells and adipocytes when cultured in lineage-specific differentiation media. Four weeks after transplanting cell line 1-11 into immunodeficient mice with beta-tricalcium phosphate (beta-TCP), the transplant produced cementum/bone-like tissues around the beta-TCP. Eight weeks after transplantation, the 1-11 cell transplant formed PDL-like structures on the surface of the beta-TCP. These data suggest that cell line 1-11 was derived from a progenitor/stem cell present in the PDL and should be very useful for studying the biology and regeneration of human periodontium.

Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. Publishing Authors By Initials

S Fujii,H Maeda,N Wada,A Tomokiyo,M Saito,A Akamine,

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Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cellular physiology

VOLUME: 215

Page Numbers: 743-9

Journal Abbreviation: J. Cell. Physiol.

ISSN: 1097-4652

DAY: 2

MONTH: Jun

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 50222

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AFFILIATION: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.

Country: United States

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MEDLINETA: J Cell Physiol

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