Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs.

Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs. Abstract Text:

K Yamamura,T Miki,N Suzuki,T Ebihara,K Kawai,Y Kumahara,T Honjo,

A fragment of the cloned gene for the mouse C epsilon chain, coding for the first, second, third, and fourth domains, has been coupled to the SV40 promotor region (pSV2-mC epsilon). About 50 copies of pSV2-mC epsilon or its PvuII-EcoRI fragments were introduced into Cos-7 cells. Expression of PvuII-EcoRI fragments of pSV2-mC epsilon was observed in about 50% of the Cos-7 cells by indirect fluorescence staining. However, no expression of circular pSV2-mC epsilon was observed. About 200 copies of linearized pSV2-mC epsilon with EcoRI were introduced into fertilized mouse eggs. Two of 78 mice born from these eggs had integrated mouse C epsilon genes. Mouse C epsilon gene was shown to be integrated in a tandem array and as intact structures without undergoing gross deletions or rearrangements, judged from the Southern blotting patterns from several restriction enzymes. The first transgenic mouse was mated to a normal male to examine whether mouse C epsilon gene were stably transmitted to progeny. Among 5 mice to which the C epsilon gene had been transmitted, one deleted 5 copies of this gene and another deleted one junction fragment, thus demonstrating relatively unstable transmission. No C epsilon mRNA was detected in the liver, kidney, brain, lung, skeletal muscle, heart, testis, or spleen of a transgenic mouse.

Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs. Publishing Authors By Initials

K Yamamura,T Miki,N Suzuki,T Ebihara,K Kawai,Y Kumahara,T Honjo,

For similar urogenital system: genitalia: germ cells: ovum: zygote research abstracts see: urogenital system: genitalia: germ cells: ovum: zygote research

PUBMED ID PMID:

MEDLINE DATE:

Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 97

Page Numbers: 333-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1985

Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs. Keywords Mesh Terms:

KEYWORDS: Zygote

MESH TERMS: genetics

Chemical & Substance for Abstract: Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs Related Publications

• Anti-androgen flutamide affects gonadal development and reproduction in medaka (Oryzias latipes).
• Application of parasagittal surface coil MRI to otoneurological diagnosis.
• Association of Killer Cell Immunoglobulin-like Receptor Genotypes with Susceptibility to Endometriosis.
• Autonomic innervations in the middle ear and pharynx.
• Biased expression of variable region gene families of the immunoglobulin heavy chain in autoimmune-prone mice.
• Biochemical and immunological characterization of the DNA binding protein (RBP-J kappa) to mouse J kappa recombination signal sequence.
• Changes in mitochondrial phosphorylative activity and adenylate energy charge of regenerating rabbit liver.
• Counter-rolling after regional destruction of the saccule by laser spot irradiation in the guinea pig.
• Effect of tributyltin on reproduction in Japanese whiting, Sillago japonica.
• Effects of estrogens on cholinergic neurons in the rat basal nucleus.
• Etiological role of adenoids upon otitis media with effusion.
• Eustachian tube function in OME patients with cleft palate. Special reference to the prognosis of otitis media with effusion.
• Genes regulated by interferon-gamma in human uterine microvascular endothelial cells.
• Greetings.
• Induction of Bip mRNA upon programmed cell death of differentiated PC12 cells as well as rat sympathetic neurons.
• Introduction of mouse C epsilon genes into Cos-7 cells and fertilized mouse eggs.
• Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein.
• Light-weight and low-cost infrared CCD eye monitoring system designed for routine vestibular clinic use.
• Loss of immunostaining of the RBP-J kappa transcription factor upon F9 cell differentiation induced by retinoic acid.
• Mitochondrial vulnerability and lipid metabolism. Brain and pancreatic mitochondria.
• Neoadjuvant chemotherapy with weekly carboplatin and paclitaxel for locally advanced cervical carcinoma.
• Pitavastatin: Protection against Neuronal Retinal Damage Induced by Ischemia-Reperfusion Injury in Rats.
• Post-ovulatory rise of endometrial CD16(-) natural killer cells: in situ proliferation of residual cells or selective recruitment from circulating peripheral blood?
• Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus.
• Purification of immunoglobulin heavy chain messenger RNA by immunoprecipitation from the mouse myeloma tumor, MOPC-31C.
• Responses and anatomical connections of peripheral vestibular neurons innervating otolith organs.
• Studies on the cell-type specific expression of RBP-L, a RBP-J family member, by replacement insertion of beta-galactosidase.
• THE EFFECTS OF ULTRAVIOLET IRRADIATION ON INDUCED FORMATION OF CATECHOL OXYGENASE IN PSEUDOMONAS EFFUSA.
• Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes.
• Usefulness of magnetic resonance imaging in diagnosing vertebro-basilar insufficiency.