Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs.

Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs. Research Abstract Details 

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Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs. Abstract Text:

K Yamamura,H Kikutani,N Takahashi,T Taga,S Akira,K Kawai,K Fukuchi,Y Kumahara,T Honjo,T Kishimoto,

A rearranged human gamma 1 immunoglobulin gene was introduced into fertilized mouse eggs. The phage Ch4A-VCE-gamma 1 was constructed by ligating an EcoRI and BglII fragment of pBR322-CESSV(CE-1) containing the VDJ region with an EcoRI and BamHI fragment of Ch4A-HIg gamma 1-10 containing the gamma 1 constant region. About 200 copies of Ch4A-VCE-gamma 1 genes were introduced into fertilized mouse eggs. Of 489 eggs injected with these genes, 319 survived and were transferred to oviducts of foster mothers. Thirtyeight mice were born and were screened for the presence of human gamma 1 immunoglobulin genes by Southern blot hybridization. Five of these 38 mice had integrated human gamma 1 immunoglobulin genes. None of the human gamma 1 copies in each mouse had undergone deletions or rearrangements as judged by the Southern blotting patterns for several restriction enzymes. Human gamma 1 gene was present in several different tissues. All the mice tested so far transmit the human gamma 1 gene to a fraction of their offspring in an autosomal dominant manner. Spleen cells from transgenic mice were analyzed for immunoglobulin production by reverse plaque assay or immunofluorescence staining of cytoplasmic immunoglobulin, but synthesis and secretion of human gamma 1 chains could not be detected. No human gamma 1 immunoglobulin mRNA was detected in the liver and spleen of a transgenic mouse. The presence of the human gamma 1 immunoglobulin gene appeared to have no effect on the expression of endogenous mouse immunoglobulin genes.

Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs. Publishing Authors By Initials

K Yamamura,H Kikutani,N Takahashi,T Taga,S Akira,K Kawai,K Fukuchi,Y Kumahara,T Honjo,T Kishimoto,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): thymidine kinase research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): thymidine kinase research

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Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 357-63

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1984

Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs. Keywords Mesh Terms:

KEYWORDS: Thymidine Kinase

MESH TERMS: deficiency

Chemical & Substance for Abstract: Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs. Information

Substance Name: DNA Restriction Enzymes

Registry Number: EC 3.1.21.-

Grant and Affiliation Information for Introduction of human gamma 1 immunoglobulin genes into fertilized mouse eggs.

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Country: JAPAN

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MEDLINETA: J Biochem

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