Intravital fluorescence microscopy in pulmonary research.

Intravital fluorescence microscopy in pulmonary research. Research Abstract Details 

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Intravital fluorescence microscopy in pulmonary research. Abstract Text:

Claudette M St Croix,Karanee Leelavanichkul,Simon C Watkins,

Over the last several years, microscopy as a scientific tool has reinvented itself evolving from a group of principally descriptive methodologies to encompass a wide range of primary tools and techniques to investigate the molecular organization of organs, tissues and cells. Advances in microscope and camera design, fluorescent dye technology, the development of fluorescent proteins as well as the advent of inexpensive powerful computers, has led to the feasibility of simultaneous sub micron resolution and quantitation of multiple concurrent molecular markers for both protein and DNA. Confocal microscopy has allowed optical sectioning and reconstruction of tissues in three dimensions. Finally, the development of multiphoton methodologies as an extension of optical sectioning microscopy has further improved the potential utility of this technology when examining living or light scattering tissues such as the lung. In order to illustrate the utility of two-photon methods in pulmonary biology, we present the application of this approach to the study of cellular trafficking in situ and to the study of pulmonary vasoregulation in an ex vivo rodent model.

Intravital fluorescence microscopy in pulmonary research. Publishing Authors By Initials

CM St Croix,K Leelavanichkul,SC Watkins,

For similar vasoconstriction research abstracts see: vasoconstriction research

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Intravital fluorescence microscopy in pulmonary research. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Advanced drug delivery reviews

VOLUME: 58

Page Numbers: 834-40

Journal Abbreviation: Adv. Drug Deliv. Rev.

ISSN: 0169-409X

DAY: 11

MONTH: 08

YEAR: 2006

Intravital fluorescence microscopy in pulmonary research. Information

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LANGUAGE: eng

NlmUniqueID: 8710523

Intravital fluorescence microscopy in pulmonary research. Keywords Mesh Terms:

KEYWORDS: Vasoconstriction

MESH TERMS: physiopathology

Chemical & Substance for Abstract: Intravital fluorescence microscopy in pulmonary research. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Intravital fluorescence microscopy in pulmonary research.

AFFILIATION: Department of Environmental and Occupational Health, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15219, USA. cls13@pitt.edu

Country: Netherlands

AGENCY: United States NCRR

GRANT: 1 U54 RR022241

ACRONYM: RR

MEDLINETA: Adv Drug Deliv Rev

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