Intravenous injection of trauma-hemorrhagic shock mesenteric lymph causes lung injury that is dependent upon activation of the inducible nitric oxide synthase pathway.

Intravenous injection of trauma-hemorrhagic shock mesenteric lymph causes lung injury that is dependent upon activation of the inducible nitric oxide synthase pathway. Research Abstract Details 

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Intravenous injection of trauma-hemorrhagic shock mesenteric lymph causes lung injury that is dependent upon activation of the inducible nitric oxide synthase pathway. Abstract Text:

Maheswari Senthil,Anthony Watkins,Dimitrios Barlos,Da-Zhong Xu,Qi Lu,Billy Abungu,Frank Caputo,Rena Feinman,Edwin A Deitch,Maheswari Senthil,Anthony Watkins,Dimitrios Barlos,Da-Zhong Xu,Qi Lu,Billy Abungu,Frank Caputo,Rena Feinman,Edwin A Deitch,

OBJECTIVE: To test the hypothesis that gut-derived factors carried in trauma-hemorrhagic shock (T/HS) lymph is sufficient to induce lung injury. Additionally, because our previous studies showed that T/HS-induced nitric oxide production was associated with lung injury, we examined whether T/HS lymph-induced lung injury occurs via an inducible nitric oxide synthase (iNOS)-dependent pathway. BACKGROUND: We have previously shown that T/HS-induced lung injury is mediated by gut-derived humoral factors carried in the mesenteric lymph. However, it remains unclear whether T/HS lymph itself is sufficient to induce lung injury, or requires the activation of other factors during the T/HS period to exert its effect. METHODS: Mesenteric lymph collected from T/HS or trauma-sham shock (T/SS) animals was injected intravenously into male rats at a rate of 1 mL/h for 3 hours. At the end of infusion, lung injury was assessed by lung permeability and lung histology. The effect of iNOS inhibition on T/HS lymph-induced lung injury was studied and this was further confirmed in iNOS knockout mice. Finally, iNOS immunohistochemistry was performed to identify the cells of origin of iNOS. RESULTS: The injection of T/HS lymph, but not sham shock lymph, caused lung injury. This was associated with increased plasma nitrite/nitrate levels as well as induction of iNOS protein in the lung, liver, and gut. Treatment with the selective iNOS inhibitor aminoguanidine prevented T/HS lymph-induced lung injury. iNOS knockout mice, but not their wild-type controls, were resistant to T/HS lymph-induced lung injury. By immunohistochemistry, neutrophils and macrophages, rather than parenchymal cells, were the source of T/HS lymph-induced lung iNOS. CONCLUSIONS: These results indicate that T/HS lymph is sufficient to induce acute lung injury and that lymph-induced lung injury occurs via an iNOS-dependent pathway.

Intravenous injection of trauma-hemorrhagic shock mesenteric lymph causes lung injury that is dependent upon activation of the inducible nitric oxide synthase pathway. Publishing Authors By Initials

M Senthil,A Watkins,D Barlos,DZ Xu,Q Lu,B Abungu,F Caputo,R Feinman,EA Deitch,M Senthil,A Watkins,D Barlos,DZ Xu,Q Lu,B Abungu,F Caputo,R Feinman,EA Deitch,

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Intravenous injection of trauma-hemorrhagic shock mesenteric lymph causes lung injury that is dependent upon activation of the inducible nitric oxide synthase pathway. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Annals of surgery

VOLUME: 246

Page Numbers: 822-30

Journal Abbreviation: Ann. Surg.

ISSN: 0003-4932

DAY: 30

MONTH: Nov

YEAR: 2007

Intravenous injection of trauma-hemorrhagic shock mesenteric lymph causes lung injury that is dependent upon activation of the inducible nitric oxide synthase pathway. Information

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LANGUAGE: eng

NlmUniqueID: 372354

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Grant and Affiliation Information for Intravenous injection of trauma-hemorrhagic shock mesenteric lymph causes lung injury that is dependent upon activation of the inducible nitric oxide synthase pathway.

AFFILIATION: Department of Surgery, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32 GM069330

ACRONYM: GM

MEDLINETA: Ann Surg

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